Averaging of viral envelope glycoprotein spikes from electron cryotomography reconstructions using Jsubtomo.

Averaging of viral envelope glycoprotein spikes from electron cryotomography reconstructions using Jsubtomo.

Enveloped viruses utilize membrane glycoproteins on their surface to mediate entry into host cells. Three-dimensional structural analysis of these glycoprotein 'spikes' is often technically challenging but important for understanding viral pathogenesis and in drug design. Here, a protocol...

Cur síos iomlán

Sonraí bibleagrafaíochta
Príomhchruthaitheoirí:	Huiskonen, J, Parsy, M, Li, S, Bitto, D, Renner, M, Bowden, T
Formáid:	Journal article
Teanga:	English
Foilsithe / Cruthaithe:	Journal of Visualized Experiments 2014

Míreanna comhchosúla

Template Matching, Alignment and Averaging of Sub-Tomograms in Electron Cryo-Tomography Reconstructions using Jsubtomo
de réir: Huiskonen, J
Foilsithe / Cruthaithe: (2014)

Electron cryotomography of Tula hantavirus suggests a unique assembly paradigm for enveloped viruses.
de réir: Huiskonen, J, et al.
Foilsithe / Cruthaithe: (2010)

Orthobunyavirus ultrastructure and the curious tripodal glycoprotein spike.
de réir: Bowden, T, et al.
Foilsithe / Cruthaithe: (2013)

Electron Cryotomography
de réir: Gavin E. Murphy, et al.
Foilsithe / Cruthaithe: (2007-10-01)

Insights into bunyavirus architecture from electron cryotomography of Uukuniemi virus.
de réir: Overby, A, et al.
Foilsithe / Cruthaithe: (2008)

Acidic pH-Induced conformations and LAMP1 binding of the Lassa virus glycoprotein spike
de réir: Li, S, et al.
Foilsithe / Cruthaithe: (2016)

Crystal structure of Venezuelan hemorrhagic fever virus fusion glycoprotein reveals a class 1 postfusion architecture with extensive glycosylation.
de réir: Parsy, M, et al.
Foilsithe / Cruthaithe: (2013)

Determination of N-linked glycosylation in viral glycoproteins by negative ion mass spectrometry and ion mobility
de réir: Bitto, D, et al.
Foilsithe / Cruthaithe: (2015)

Electron cryotomography of measles virus reveals how matrix protein coats the ribonucleocapsid within intact virions.
de réir: Liljeroos, L, et al.
Foilsithe / Cruthaithe: (2011)

Serial Cryomicrotomy of Saccharomyces cerevisiae for Serial Electron Cryotomography
de réir: Cai Tong Ng, et al.
Foilsithe / Cruthaithe: (2020-11-01)

Structural transitions of the conserved and metastable hantaviral glycoprotein envelope
de réir: Rissanen, I, et al.
Foilsithe / Cruthaithe: (2017)

Acidic pH-Induced Conformations and LAMP1 Binding of the Lassa Virus Glycoprotein Spike.
de réir: Sai Li, et al.
Foilsithe / Cruthaithe: (2016-02-01)

Structure of a phleboviral envelope glycoprotein reveals a consolidated model of membrane fusion.
de réir: Halldorsson, S, et al.
Foilsithe / Cruthaithe: (2016)

DNA Nanotags for Multiplexed Single-Particle Electron Microscopy and In Situ Electron Cryotomography
de réir: Yuanfang Chen, et al.
Foilsithe / Cruthaithe: (2024-12-01)

Towards high-throughput in situ structural biology using electron cryotomography
de réir: Böhning, J, et al.
Foilsithe / Cruthaithe: (2020)

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography
de réir: Silvester, E, et al.
Foilsithe / Cruthaithe: (2021)

Locating macromolecules and determining structures inside bacterial cells using electron cryotomography
de réir: Melia, C, et al.
Foilsithe / Cruthaithe: (2018)

Mechanisms of microtubule dynamics and force generation examined with computational modeling and electron cryotomography
de réir: Nikita B. Gudimchuk, et al.
Foilsithe / Cruthaithe: (2020-07-01)

3-D ultrastructure of O. tauri: electron cryotomography of an entire eukaryotic cell.
de réir: Gregory P Henderson, et al.
Foilsithe / Cruthaithe: (2007-08-01)

HIV-1 Envelope Glycoprotein Trafficking and Viral Transmission
de réir: Melissa Victoria Fernandez, et al.
Foilsithe / Cruthaithe: (2020-06-01)

Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments: implications of crowding.
de réir: Grünewald, K, et al.
Foilsithe / Cruthaithe: (2003)

Electron cryo-microscopy and single-particle averaging of Rift Valley fever virus: evidence for GN-GC glycoprotein heterodimers.
de réir: Huiskonen, J, et al.
Foilsithe / Cruthaithe: (2009)

Insights into centriole geometry revealed by cryotomography of doublet and triplet centrioles
de réir: Garrett A Greenan, et al.
Foilsithe / Cruthaithe: (2018-08-01)

HIV-1 Diversity in the Envelope Glycoproteins: Implications for Viral Entry Inhibition
de réir: Sabrina E. M. Almeida, et al.
Foilsithe / Cruthaithe: (2013-02-01)

Palmitoylation of the Bovine Foamy Virus Envelope Glycoprotein Is Required for Viral Replication
de réir: Keli Chai, et al.
Foilsithe / Cruthaithe: (2020-12-01)

Co-assembly of viral envelope glycoproteins regulates their polarized sorting in neurons.
de réir: Rafael Mattera, et al.
Foilsithe / Cruthaithe: (2014-05-01)

Apoptotic killing of HIV-1-infected macrophages is subverted by the viral envelope glycoprotein.
de réir: Simon Swingler, et al.
Foilsithe / Cruthaithe: (2007-09-01)

Envelope Glycoprotein of Arenaviruses
de réir: Antonella Pasquato, et al.
Foilsithe / Cruthaithe: (2012-10-01)

MARCH8 Targets Cytoplasmic Lysine Residues of Various Viral Envelope Glycoproteins
de réir: Yanzhao Zhang, et al.
Foilsithe / Cruthaithe: (2022-02-01)

Dynamic organization of Herpesvirus glycoproteins on the viral envelope revealed by super-resolution microscopy.
de réir: Frauke Beilstein, et al.
Foilsithe / Cruthaithe: (2019-12-01)

A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses
de réir: Touraj Aligholipour Farzani, et al.
Foilsithe / Cruthaithe: (2020-12-01)

Enhancing anti-viral neutralization response to immunization with HIV-1 envelope glycoprotein immunogens
de réir: Shamim Ahmed, et al.
Foilsithe / Cruthaithe: (2023-11-01)

Identifying the Viral Genes Encoding Envelope Glycoproteins for Differentiation of Cyprinid herpesvirus 3 Isolates
de réir: Se Chang Park, et al.
Foilsithe / Cruthaithe: (2013-01-01)

Increased functional stability and homogeneity of viral envelope spikes through directed evolution.
de réir: Daniel P Leaman, et al.
Foilsithe / Cruthaithe: (2013-02-01)

Cells under siege: viral glycoprotein interactions at the cell surface.
de réir: Bowden, T, et al.
Foilsithe / Cruthaithe: (2011)

Cells under siege: Viral glycoprotein interactions at the cell surface
de réir: Bowden, T, et al.
Foilsithe / Cruthaithe: (2011)

Combined 1H-detected solid-state NMR spectroscopy and electron cryotomography to study membrane proteins across resolutions in native environments
de réir: Baker, L, et al.
Foilsithe / Cruthaithe: (2017)

Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly
de réir: Lesley J. Calder, et al.
Foilsithe / Cruthaithe: (2022-11-01)

The structure of the bacteriophage PRD1 spike sheds light on the evolution of viral capsid architecture.
de réir: Merckel, M, et al.
Foilsithe / Cruthaithe: (2005)

Oncolytic herpes simplex virus tumor targeting and neutralization escape by engineering viral envelope glycoproteins
de réir: Xiao-Qin Liu, et al.
Foilsithe / Cruthaithe: (2018-01-01)