Spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart
<p>The embryonic epicardium, originating from the proepicardial organ (PEO), provides a source of multipotent progenitors for cardiac lineages, including pericytes, fibroblasts, and vascular smooth muscle cells. Maximizing the regenerative capacity of the adult epicardium depends on recapitula...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2020
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| _version_ | 1826298838981279744 |
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| author | Lupu, I-E Redpath, AN Smart, N |
| author_facet | Lupu, I-E Redpath, AN Smart, N |
| author_sort | Lupu, I-E |
| collection | OXFORD |
| description | <p>The embryonic epicardium, originating from the proepicardial organ (PEO), provides a source of multipotent progenitors for cardiac lineages, including pericytes, fibroblasts, and vascular smooth muscle cells. Maximizing the regenerative capacity of the adult epicardium depends on recapitulating embryonic cell fates. The potential of the epicardium to contribute coronary endothelium is unclear, due to conflicting Cre-based lineage trace data. Controversy also surrounds when epicardial cell fate becomes restricted. Here, we systematically investigate expression of five widely used epicardial markers, <em>Wt1</em>, <em>Tcf21</em>, <em>Tbx18</em>, <em>Sema3d</em>, and <em>Scx</em>, over the course of development. We show overlap of markers in all PEO and epicardial cells until E13.5, and find no evidence for discrete proepicardial sub-compartments that might contribute coronary endothelium via the epicardial layer. Our findings clarify a number of prevailing discrepancies and support the notion that epicardium-derived cell fate, to form fibroblasts or mural cells, is specified after epithelial-mesenchymal transition, not pre-determined within the PEO.</p> |
| first_indexed | 2024-03-07T04:52:54Z |
| format | Journal article |
| id | oxford-uuid:d5953900-1eba-4cf6-8ed0-1ddbccff5298 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:52:54Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:d5953900-1eba-4cf6-8ed0-1ddbccff52982022-03-27T08:27:04ZSpatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heartJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d5953900-1eba-4cf6-8ed0-1ddbccff5298EnglishSymplectic ElementsElsevier2020Lupu, I-ERedpath, ANSmart, N<p>The embryonic epicardium, originating from the proepicardial organ (PEO), provides a source of multipotent progenitors for cardiac lineages, including pericytes, fibroblasts, and vascular smooth muscle cells. Maximizing the regenerative capacity of the adult epicardium depends on recapitulating embryonic cell fates. The potential of the epicardium to contribute coronary endothelium is unclear, due to conflicting Cre-based lineage trace data. Controversy also surrounds when epicardial cell fate becomes restricted. Here, we systematically investigate expression of five widely used epicardial markers, <em>Wt1</em>, <em>Tcf21</em>, <em>Tbx18</em>, <em>Sema3d</em>, and <em>Scx</em>, over the course of development. We show overlap of markers in all PEO and epicardial cells until E13.5, and find no evidence for discrete proepicardial sub-compartments that might contribute coronary endothelium via the epicardial layer. Our findings clarify a number of prevailing discrepancies and support the notion that epicardium-derived cell fate, to form fibroblasts or mural cells, is specified after epithelial-mesenchymal transition, not pre-determined within the PEO.</p> |
| spellingShingle | Lupu, I-E Redpath, AN Smart, N Spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart |
| title | Spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart |
| title_full | Spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart |
| title_fullStr | Spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart |
| title_full_unstemmed | Spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart |
| title_short | Spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart |
| title_sort | spatiotemporal analysis reveals overlap of key proepicardial markers in the developing murine heart |
| work_keys_str_mv | AT lupuie spatiotemporalanalysisrevealsoverlapofkeyproepicardialmarkersinthedevelopingmurineheart AT redpathan spatiotemporalanalysisrevealsoverlapofkeyproepicardialmarkersinthedevelopingmurineheart AT smartn spatiotemporalanalysisrevealsoverlapofkeyproepicardialmarkersinthedevelopingmurineheart |