Engineering cytochrome P450cam into an alkane hydroxylase

The haem monooxygenase cytochrome P450cam from Pseudomonas putida has been engineered into an alkane hydroxylase. Active site amino acid residues were substituted with residues that have bulkier and more hydrophobic side-chains. The residues F87, Y96, V247 and V396, which are further away from the h...

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Detalhes bibliográficos
Principais autores: Bell, S, Orton, E, Boyd, H, Stevenson, J, Riddle, A, Campbell, S, Wong, L
Formato: Journal article
Idioma:English
Publicado em: 2003