Shigella MreB promotes polar IcsA positioning for actin tail formation
Shigella is a paradigm to address key issues of cell and infection biology. Polar localisation of the Shigella autotransporter protein IcsA is essential for actin tail formation, yet how proteins are targeted to the bacterial cell pole is poorly understood. The bacterial actin homologue MreB has bee...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Company of Biologists
2019
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| _version_ | 1826342841859702784 |
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| author | Krokowski, S Atwal, S Lobato-Márquez, D Chastanet, A Carballido-López, R Salje, J Mostowy, S |
| author_facet | Krokowski, S Atwal, S Lobato-Márquez, D Chastanet, A Carballido-López, R Salje, J Mostowy, S |
| author_sort | Krokowski, S |
| collection | OXFORD |
| description | Shigella is a paradigm to address key issues of cell and infection biology. Polar localisation of the Shigella autotransporter protein IcsA is essential for actin tail formation, yet how proteins are targeted to the bacterial cell pole is poorly understood. The bacterial actin homologue MreB has been extensively studied in broth culture using model organisms including Escherichia coli, Bacillus subtilis and Caulobacter crescentus, but has never been visualised in rod-shaped pathogenic bacteria during infection of host cells. Here, using single-cell analysis of intracellular Shigella, we discover that MreB accumulates at the cell pole of bacteria forming actin tails, where it colocalises with IcsA. Pharmacologic inhibition of host cell actin polymerisation and genetic deletion of IcsA is used to show, respectively, that MreB polarisation precedes actin tail formation and IcsA polarisation. Finally, exploiting MreB inhibitors A22 and MP265, we demonstrate that MreB polymerisation can support actin tail formation. We conclude that Shigella MreB promotes polar IcsA positioning for actin tail formation, and suggest that understanding the bacterial cytoskeleton during host-pathogen interactions can inspire development of new therapeutic regimes for infection control. |
| first_indexed | 2024-03-07T04:53:40Z |
| format | Journal article |
| id | oxford-uuid:d5d53d9f-fbe5-4b9d-8193-7411f8491ab5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:53:40Z |
| publishDate | 2019 |
| publisher | Company of Biologists |
| record_format | dspace |
| spelling | oxford-uuid:d5d53d9f-fbe5-4b9d-8193-7411f8491ab52022-03-27T08:28:55ZShigella MreB promotes polar IcsA positioning for actin tail formationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d5d53d9f-fbe5-4b9d-8193-7411f8491ab5EnglishSymplectic Elements at OxfordCompany of Biologists2019Krokowski, SAtwal, SLobato-Márquez, DChastanet, ACarballido-López, RSalje, JMostowy, SShigella is a paradigm to address key issues of cell and infection biology. Polar localisation of the Shigella autotransporter protein IcsA is essential for actin tail formation, yet how proteins are targeted to the bacterial cell pole is poorly understood. The bacterial actin homologue MreB has been extensively studied in broth culture using model organisms including Escherichia coli, Bacillus subtilis and Caulobacter crescentus, but has never been visualised in rod-shaped pathogenic bacteria during infection of host cells. Here, using single-cell analysis of intracellular Shigella, we discover that MreB accumulates at the cell pole of bacteria forming actin tails, where it colocalises with IcsA. Pharmacologic inhibition of host cell actin polymerisation and genetic deletion of IcsA is used to show, respectively, that MreB polarisation precedes actin tail formation and IcsA polarisation. Finally, exploiting MreB inhibitors A22 and MP265, we demonstrate that MreB polymerisation can support actin tail formation. We conclude that Shigella MreB promotes polar IcsA positioning for actin tail formation, and suggest that understanding the bacterial cytoskeleton during host-pathogen interactions can inspire development of new therapeutic regimes for infection control. |
| spellingShingle | Krokowski, S Atwal, S Lobato-Márquez, D Chastanet, A Carballido-López, R Salje, J Mostowy, S Shigella MreB promotes polar IcsA positioning for actin tail formation |
| title | Shigella MreB promotes polar IcsA positioning for actin tail formation |
| title_full | Shigella MreB promotes polar IcsA positioning for actin tail formation |
| title_fullStr | Shigella MreB promotes polar IcsA positioning for actin tail formation |
| title_full_unstemmed | Shigella MreB promotes polar IcsA positioning for actin tail formation |
| title_short | Shigella MreB promotes polar IcsA positioning for actin tail formation |
| title_sort | shigella mreb promotes polar icsa positioning for actin tail formation |
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