Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities.
Genomic alterations and abnormal expression of the FHIT gene at 3p14.2 have been observed in cell lines and primary tumors of the lung. To correlate FHIT locus DNA and RNA lesions with effects on Fhit protein expression, we have analyzed 11 lung cancer cell lines, 15 small cell lung carcinomas, and...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1997
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| _version_ | 1797097384065368064 |
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| author | Sozzi, G Tornielli, S Tagliabue, E Sard, L Pezzella, F Pastorino, U Minoletti, F Pilotti, S Ratcliffe, C Veronese, M Goldstraw, P Huebner, K Croce, C Pierotti, M |
| author_facet | Sozzi, G Tornielli, S Tagliabue, E Sard, L Pezzella, F Pastorino, U Minoletti, F Pilotti, S Ratcliffe, C Veronese, M Goldstraw, P Huebner, K Croce, C Pierotti, M |
| author_sort | Sozzi, G |
| collection | OXFORD |
| description | Genomic alterations and abnormal expression of the FHIT gene at 3p14.2 have been observed in cell lines and primary tumors of the lung. To correlate FHIT locus DNA and RNA lesions with effects on Fhit protein expression, we have analyzed 11 lung cancer cell lines, 15 small cell lung carcinomas, and 38 pairs of non-small cell primary tumors and bronchial mucosa specimens by molecular genetic and immunocytochemical methods. Using specific antibodies against the Fhit protein, we observed concordance between RNA abnormalities and lack of Fhit protein expression in lung tumors and cell lines. In addition, absence of Fhit protein in some precancerous dysplastic lesions suggested that FHIT inactivation may occur at an early phase of lung carcinogenesis. |
| first_indexed | 2024-03-07T04:54:43Z |
| format | Journal article |
| id | oxford-uuid:d632679d-9652-415f-b45d-39053e144af3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:54:43Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:d632679d-9652-415f-b45d-39053e144af32022-03-27T08:31:44ZAbsence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d632679d-9652-415f-b45d-39053e144af3EnglishSymplectic Elements at Oxford1997Sozzi, GTornielli, STagliabue, ESard, LPezzella, FPastorino, UMinoletti, FPilotti, SRatcliffe, CVeronese, MGoldstraw, PHuebner, KCroce, CPierotti, MGenomic alterations and abnormal expression of the FHIT gene at 3p14.2 have been observed in cell lines and primary tumors of the lung. To correlate FHIT locus DNA and RNA lesions with effects on Fhit protein expression, we have analyzed 11 lung cancer cell lines, 15 small cell lung carcinomas, and 38 pairs of non-small cell primary tumors and bronchial mucosa specimens by molecular genetic and immunocytochemical methods. Using specific antibodies against the Fhit protein, we observed concordance between RNA abnormalities and lack of Fhit protein expression in lung tumors and cell lines. In addition, absence of Fhit protein in some precancerous dysplastic lesions suggested that FHIT inactivation may occur at an early phase of lung carcinogenesis. |
| spellingShingle | Sozzi, G Tornielli, S Tagliabue, E Sard, L Pezzella, F Pastorino, U Minoletti, F Pilotti, S Ratcliffe, C Veronese, M Goldstraw, P Huebner, K Croce, C Pierotti, M Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities. |
| title | Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities. |
| title_full | Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities. |
| title_fullStr | Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities. |
| title_full_unstemmed | Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities. |
| title_short | Absence of Fhit protein in primary lung tumors and cell lines with FHIT gene abnormalities. |
| title_sort | absence of fhit protein in primary lung tumors and cell lines with fhit gene abnormalities |
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