International multicenter examination of MOG antibody assays
<p><strong>Objective</strong> To compare the reproducibility of 11 antibody assays for immunoglobulin (Ig) G and IgM myelin oligodendrocyte glycoprotein antibodies (MOG-IgG and MOG-IgM) from 5 international centers.</p> <p><strong>Methods</strong> The follo...
| Asıl Yazarlar: | , , , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Lippincott, Williams and Wilkins
2020
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| _version_ | 1826298988562743296 |
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| author | Reindl, M Schanda, K Woodhall, M Ramberger, M Leite, MI Palace, J Irani, SR Waters, P |
| author_facet | Reindl, M Schanda, K Woodhall, M Ramberger, M Leite, MI Palace, J Irani, SR Waters, P |
| author_sort | Reindl, M |
| collection | OXFORD |
| description | <p><strong>Objective</strong> To compare the reproducibility of 11 antibody assays for immunoglobulin (Ig) G and IgM myelin oligodendrocyte glycoprotein antibodies (MOG-IgG and MOG-IgM) from 5 international centers.</p>
<p><strong>Methods</strong> The following samples were analyzed: MOG-IgG clearly positive sera (n = 39), MOG-IgG low positive sera (n = 39), borderline negative sera (n = 13), clearly negative sera (n = 40), and healthy blood donors (n = 30). As technical controls, 18 replicates (9 MOG-IgG positive and 9 negative) were included. All samples and controls were recoded, aliquoted, and distributed to the 5 testing centers, which performed the following antibody assays: 5 live and 1 fixed immunofluorescence cell-based assays (CBA-IF, 5 MOG-IgG, and 1 MOG-IgM), 3 live flow cytometry cell-based assays (CBA-FACS, all MOG-IgG), and 2 ELISAs (both MOG-IgG).</p>
<p><strong>Results</strong> We found excellent agreement (96%) between the live CBAs for MOG-IgG for samples previously identified as clearly positive or negative from 4 different national testing centers. The agreement was lower with fixed CBA-IF (90%), and the ELISA showed no concordance with CBAs for detection of human MOG-IgG. All CBAs showed excellent interassay reproducibility. The agreement of MOG-IgG CBAs for borderline negative (77%) and particularly low positive (33%) samples was less good. Finally, most samples from healthy blood donors (97%) were negative for MOG-IgG in all CBAs.</p>
<p><strong>Conclusions</strong> Live MOG-IgG CBAs showed excellent agreement for high positive and negative samples at 3 international testing centers. Low positive samples were more frequently discordant than in a similar comparison of aquaporin-4 antibody assays. Further research is needed to improve international standardization for clinical care.</p> |
| first_indexed | 2024-03-07T04:55:05Z |
| format | Journal article |
| id | oxford-uuid:d6511134-a8db-4f3d-b5cb-26ec0118daa3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:55:05Z |
| publishDate | 2020 |
| publisher | Lippincott, Williams and Wilkins |
| record_format | dspace |
| spelling | oxford-uuid:d6511134-a8db-4f3d-b5cb-26ec0118daa32022-03-27T08:32:39ZInternational multicenter examination of MOG antibody assaysJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d6511134-a8db-4f3d-b5cb-26ec0118daa3EnglishSymplectic Elements at OxfordLippincott, Williams and Wilkins2020Reindl, MSchanda, KWoodhall, MRamberger, MLeite, MIPalace, JIrani, SRWaters, P<p><strong>Objective</strong> To compare the reproducibility of 11 antibody assays for immunoglobulin (Ig) G and IgM myelin oligodendrocyte glycoprotein antibodies (MOG-IgG and MOG-IgM) from 5 international centers.</p> <p><strong>Methods</strong> The following samples were analyzed: MOG-IgG clearly positive sera (n = 39), MOG-IgG low positive sera (n = 39), borderline negative sera (n = 13), clearly negative sera (n = 40), and healthy blood donors (n = 30). As technical controls, 18 replicates (9 MOG-IgG positive and 9 negative) were included. All samples and controls were recoded, aliquoted, and distributed to the 5 testing centers, which performed the following antibody assays: 5 live and 1 fixed immunofluorescence cell-based assays (CBA-IF, 5 MOG-IgG, and 1 MOG-IgM), 3 live flow cytometry cell-based assays (CBA-FACS, all MOG-IgG), and 2 ELISAs (both MOG-IgG).</p> <p><strong>Results</strong> We found excellent agreement (96%) between the live CBAs for MOG-IgG for samples previously identified as clearly positive or negative from 4 different national testing centers. The agreement was lower with fixed CBA-IF (90%), and the ELISA showed no concordance with CBAs for detection of human MOG-IgG. All CBAs showed excellent interassay reproducibility. The agreement of MOG-IgG CBAs for borderline negative (77%) and particularly low positive (33%) samples was less good. Finally, most samples from healthy blood donors (97%) were negative for MOG-IgG in all CBAs.</p> <p><strong>Conclusions</strong> Live MOG-IgG CBAs showed excellent agreement for high positive and negative samples at 3 international testing centers. Low positive samples were more frequently discordant than in a similar comparison of aquaporin-4 antibody assays. Further research is needed to improve international standardization for clinical care.</p> |
| spellingShingle | Reindl, M Schanda, K Woodhall, M Ramberger, M Leite, MI Palace, J Irani, SR Waters, P International multicenter examination of MOG antibody assays |
| title | International multicenter examination of MOG antibody assays |
| title_full | International multicenter examination of MOG antibody assays |
| title_fullStr | International multicenter examination of MOG antibody assays |
| title_full_unstemmed | International multicenter examination of MOG antibody assays |
| title_short | International multicenter examination of MOG antibody assays |
| title_sort | international multicenter examination of mog antibody assays |
| work_keys_str_mv | AT reindlm internationalmulticenterexaminationofmogantibodyassays AT schandak internationalmulticenterexaminationofmogantibodyassays AT woodhallm internationalmulticenterexaminationofmogantibodyassays AT rambergerm internationalmulticenterexaminationofmogantibodyassays AT leitemi internationalmulticenterexaminationofmogantibodyassays AT palacej internationalmulticenterexaminationofmogantibodyassays AT iranisr internationalmulticenterexaminationofmogantibodyassays AT watersp internationalmulticenterexaminationofmogantibodyassays |