Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli.

Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli.

We report here modifications of human beta-globin PAC clones by homologous recombination in Escherichia coli DH10B, utilising a plasmid temperature sensitive for replication, the recA gene and a wild-type copy of the rpsL gene which allows for an efficient selection for plasmid loss in this host. Hi...

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Bibliographic Details
Main Authors:	Imam, A, Patrinos, G, de Krom, M, Bottardi, S, Janssens, R, Katsantoni, E, Wai, A, Sherratt, D, Grosveld, F
Format:	Journal article
Language:	English
Published:	2000

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