Production of soluble alphabeta T-cell receptor heterodimers suitable for biophysical analysis of ligand binding.
A method to produce alphabeta T-cell receptors (TCRs) in a soluble form suitable for biophysical analysis was devised involving in vitro refolding of a TCR fusion protein. Polypeptides corresponding to the variable and constant domains of each chain of a human and a murine receptor, fused to a coile...
Main Authors: | Willcox, B, Gao, G, Wyer, JR, O'Callaghan, C, Boulter, J, Jones, E, Van Der Merwe, P, Bell, J, Jakobsen, B |
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Format: | Journal article |
Language: | English |
Published: |
1999
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