The nucleus: a highly organized but dynamic structure.
The nucleus in plants and animals is a highly structured organelle containing several well-defined subregions or suborganelles. These include the nucleolus, interphase chromosome territories and coiled bodies. We have visualized transcription sites in plants at both light- and electron-microscopy le...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2000
|
| _version_ | 1797097642061201408 |
|---|---|
| author | Gonzalez-Melendi, P Beven, A Boudonck, K Abranches, R Wells, B Dolan, L Shaw, P |
| author_facet | Gonzalez-Melendi, P Beven, A Boudonck, K Abranches, R Wells, B Dolan, L Shaw, P |
| author_sort | Gonzalez-Melendi, P |
| collection | OXFORD |
| description | The nucleus in plants and animals is a highly structured organelle containing several well-defined subregions or suborganelles. These include the nucleolus, interphase chromosome territories and coiled bodies. We have visualized transcription sites in plants at both light- and electron-microscopy level by the incorporation of BrUTP. In the nucleolus many dispersed foci are revealed within the dense fibrillar component, each of which probably corresponds to a single gene copy. In the nucleoplasm there are also many dispersed foci of transcription, but not enough to correspond to one site per transcribed gene. We have shown that in wheat, and probably many other plant species, interphase chromosome territories are organized in a very regular way, with all the chromosomes in the Rabl configuration, all the centromeres clustered at the nuclear membrane and all the telomeres located at the nuclear membrane on the opposite side of the nucleus. However, despite this regular, polarized structure, there is no sign of polarization of transcription sites, or of any preferred location for them with respect to chromosome territorial boundaries. The nucleus is also highly dynamic. As an example, we have shown by the use of a green fluorescent protein fusion to the spliceosomal protein U2B" that coiled bodies move and coalesce within the nucleus, and may act as transport structures within the nucleus and nucleolus. |
| first_indexed | 2024-03-07T04:58:23Z |
| format | Journal article |
| id | oxford-uuid:d768a090-55ed-41da-aad9-a27836b492ed |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:58:23Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:d768a090-55ed-41da-aad9-a27836b492ed2022-03-27T08:40:49ZThe nucleus: a highly organized but dynamic structure.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d768a090-55ed-41da-aad9-a27836b492edEnglishSymplectic Elements at Oxford2000Gonzalez-Melendi, PBeven, ABoudonck, KAbranches, RWells, BDolan, LShaw, PThe nucleus in plants and animals is a highly structured organelle containing several well-defined subregions or suborganelles. These include the nucleolus, interphase chromosome territories and coiled bodies. We have visualized transcription sites in plants at both light- and electron-microscopy level by the incorporation of BrUTP. In the nucleolus many dispersed foci are revealed within the dense fibrillar component, each of which probably corresponds to a single gene copy. In the nucleoplasm there are also many dispersed foci of transcription, but not enough to correspond to one site per transcribed gene. We have shown that in wheat, and probably many other plant species, interphase chromosome territories are organized in a very regular way, with all the chromosomes in the Rabl configuration, all the centromeres clustered at the nuclear membrane and all the telomeres located at the nuclear membrane on the opposite side of the nucleus. However, despite this regular, polarized structure, there is no sign of polarization of transcription sites, or of any preferred location for them with respect to chromosome territorial boundaries. The nucleus is also highly dynamic. As an example, we have shown by the use of a green fluorescent protein fusion to the spliceosomal protein U2B" that coiled bodies move and coalesce within the nucleus, and may act as transport structures within the nucleus and nucleolus. |
| spellingShingle | Gonzalez-Melendi, P Beven, A Boudonck, K Abranches, R Wells, B Dolan, L Shaw, P The nucleus: a highly organized but dynamic structure. |
| title | The nucleus: a highly organized but dynamic structure. |
| title_full | The nucleus: a highly organized but dynamic structure. |
| title_fullStr | The nucleus: a highly organized but dynamic structure. |
| title_full_unstemmed | The nucleus: a highly organized but dynamic structure. |
| title_short | The nucleus: a highly organized but dynamic structure. |
| title_sort | nucleus a highly organized but dynamic structure |
| work_keys_str_mv | AT gonzalezmelendip thenucleusahighlyorganizedbutdynamicstructure AT bevena thenucleusahighlyorganizedbutdynamicstructure AT boudonckk thenucleusahighlyorganizedbutdynamicstructure AT abranchesr thenucleusahighlyorganizedbutdynamicstructure AT wellsb thenucleusahighlyorganizedbutdynamicstructure AT dolanl thenucleusahighlyorganizedbutdynamicstructure AT shawp thenucleusahighlyorganizedbutdynamicstructure AT gonzalezmelendip nucleusahighlyorganizedbutdynamicstructure AT bevena nucleusahighlyorganizedbutdynamicstructure AT boudonckk nucleusahighlyorganizedbutdynamicstructure AT abranchesr nucleusahighlyorganizedbutdynamicstructure AT wellsb nucleusahighlyorganizedbutdynamicstructure AT dolanl nucleusahighlyorganizedbutdynamicstructure AT shawp nucleusahighlyorganizedbutdynamicstructure |