Enzymatic incorporation and fluorescent labelling of cyclooctyne-modified deoxyuridine triphosphates in DNA.
The amino group of 5-aminopropargyl-2'-deoxyuridine-5'-triphosphate was labelled with dibenzocyclooctyne (DIBO) and two derivatives of bicyclo [6.1.0] non-4-yne (BCN) with short and long linkers to produce three different cycloalkyne-modified deoxyuridine triphosphates. BCN was successfull...
Những tác giả chính: | Ren, X, Gerowska, M, El-Sagheer, A, Brown, T |
---|---|
Định dạng: | Journal article |
Ngôn ngữ: | English |
Được phát hành: |
Elsevier
2014
|
Những quyển sách tương tự
-
Synthesis and Enzymatic Incorporation of Modified Deoxyuridine Triphosphates
Bằng: Erkai Liu, et al.
Được phát hành: (2015-07-01) -
Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Bằng: Ren, X, et al.
Được phát hành: (2015) -
Synthesis of Polyanionic C5-Modified 2′-Deoxyuridine and 2′-Deoxycytidine-5′-Triphosphates and Their Properties as Substrates for DNA Polymerases
Bằng: Claire Dutson, et al.
Được phát hành: (2021-04-01) -
Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes.
Bằng: Ren, X, et al.
Được phát hành: (2016) -
Structural dynamics of human deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase)
Bằng: Ravdna Sarre, et al.
Được phát hành: (2024-10-01)