The type 1 insulin-like growth factor receptor is over-expressed in bladder cancer.
OBJECTIVE: To analyse bladder cancer biopsies and investigate the pattern of expression of the type 1 insulin-like growth factor receptor (IGF1R), a receptor tyrosine kinase that mediates tumour cell proliferation, motility and protection from apoptosis. MATERIALS AND METHODS: Formalin-fixed specime...
| Autori principali: | , , , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
2007
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| _version_ | 1826299247604006912 |
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| author | Rochester, M Patel, N Turney, B Davies, DR Roberts, I Crew, J Protheroe, A Macaulay, V |
| author_facet | Rochester, M Patel, N Turney, B Davies, DR Roberts, I Crew, J Protheroe, A Macaulay, V |
| author_sort | Rochester, M |
| collection | OXFORD |
| description | OBJECTIVE: To analyse bladder cancer biopsies and investigate the pattern of expression of the type 1 insulin-like growth factor receptor (IGF1R), a receptor tyrosine kinase that mediates tumour cell proliferation, motility and protection from apoptosis. MATERIALS AND METHODS: Formalin-fixed specimens of bladder cancer (40 whole-mount, 80 cores on a tumour microarray) and normal bladder (15 samples) were stained immunohistochemically for the IGF1R. The IGF1R expression was also measured by quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) on RNA extracted from fresh frozen bladder cancers (61) and benign bladder (12). RESULTS: Of the 15 samples of normal bladder, 14 showed negligible (1+) or light (2+) IGF1R immunostaining. By contrast moderate (3+) or heavy (4+) staining for IGF1R was detected in 89 (74%) of the 120 samples of malignant urothelium. Q-RT-PCR showed significantly higher levels of steady-state IGF1R mRNA in tumours (all cases, Ta-T4) than in normal bladder (P < 0.05), indicating up-regulation at the transcriptional level. This difference was particularly evident when comparing normal urothelium with superficial (Ta-T1) or invasive (T2-4) tumours; only the latter showed significant IGF1R over-expression at the RNA level (P < 0.05 vs normal bladder). CONCLUSION: The IGF1R is up-regulated in bladder cancer compared with non-malignant bladder, and might contribute to a propensity for invasion. |
| first_indexed | 2024-03-07T04:59:01Z |
| format | Journal article |
| id | oxford-uuid:d79e6693-ba9c-43a8-a90f-e5a82a926d52 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:59:01Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:d79e6693-ba9c-43a8-a90f-e5a82a926d522022-03-27T08:42:24ZThe type 1 insulin-like growth factor receptor is over-expressed in bladder cancer.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d79e6693-ba9c-43a8-a90f-e5a82a926d52EnglishSymplectic Elements at Oxford2007Rochester, MPatel, NTurney, BDavies, DRRoberts, ICrew, JProtheroe, AMacaulay, VOBJECTIVE: To analyse bladder cancer biopsies and investigate the pattern of expression of the type 1 insulin-like growth factor receptor (IGF1R), a receptor tyrosine kinase that mediates tumour cell proliferation, motility and protection from apoptosis. MATERIALS AND METHODS: Formalin-fixed specimens of bladder cancer (40 whole-mount, 80 cores on a tumour microarray) and normal bladder (15 samples) were stained immunohistochemically for the IGF1R. The IGF1R expression was also measured by quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) on RNA extracted from fresh frozen bladder cancers (61) and benign bladder (12). RESULTS: Of the 15 samples of normal bladder, 14 showed negligible (1+) or light (2+) IGF1R immunostaining. By contrast moderate (3+) or heavy (4+) staining for IGF1R was detected in 89 (74%) of the 120 samples of malignant urothelium. Q-RT-PCR showed significantly higher levels of steady-state IGF1R mRNA in tumours (all cases, Ta-T4) than in normal bladder (P < 0.05), indicating up-regulation at the transcriptional level. This difference was particularly evident when comparing normal urothelium with superficial (Ta-T1) or invasive (T2-4) tumours; only the latter showed significant IGF1R over-expression at the RNA level (P < 0.05 vs normal bladder). CONCLUSION: The IGF1R is up-regulated in bladder cancer compared with non-malignant bladder, and might contribute to a propensity for invasion. |
| spellingShingle | Rochester, M Patel, N Turney, B Davies, DR Roberts, I Crew, J Protheroe, A Macaulay, V The type 1 insulin-like growth factor receptor is over-expressed in bladder cancer. |
| title | The type 1 insulin-like growth factor receptor is over-expressed in bladder cancer. |
| title_full | The type 1 insulin-like growth factor receptor is over-expressed in bladder cancer. |
| title_fullStr | The type 1 insulin-like growth factor receptor is over-expressed in bladder cancer. |
| title_full_unstemmed | The type 1 insulin-like growth factor receptor is over-expressed in bladder cancer. |
| title_short | The type 1 insulin-like growth factor receptor is over-expressed in bladder cancer. |
| title_sort | type 1 insulin like growth factor receptor is over expressed in bladder cancer |
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