Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley.
Barley powdery mildew, Erysiphe graminis f.sp. hordei, is an obligate biotrophic pathogen and as such cannot complete its life cycle without a living host. The inability to transform this fungus and manipulate its genome has constrained research towards understanding its life cycle and pathogenicity...
| Main Authors: | , , |
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| Format: | Journal article |
| Sprog: | English |
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2000
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| _version_ | 1826299249763024896 |
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| author | Chaure, P Gurr, S Spanu, P |
| author_facet | Chaure, P Gurr, S Spanu, P |
| author_sort | Chaure, P |
| collection | OXFORD |
| description | Barley powdery mildew, Erysiphe graminis f.sp. hordei, is an obligate biotrophic pathogen and as such cannot complete its life cycle without a living host. The inability to transform this fungus and manipulate its genome has constrained research towards understanding its life cycle and pathogenicity. Here we describe an in planta transformation system based on delivery of DNA using a gold-particle gun and selection using benomyl or bialaphos. Using this method, we consistently obtained stable transformants with efficiencies comparable to other filamentous fungi. Stable expression of the beta-glucuronidase in E. graminis was demonstrated by co-transforming the uidA gene with the selectable markers. |
| first_indexed | 2024-03-07T04:59:03Z |
| format | Journal article |
| id | oxford-uuid:d7a0deaf-de2e-4b18-8ca7-97cd1613eb21 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T04:59:03Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:d7a0deaf-de2e-4b18-8ca7-97cd1613eb212022-03-27T08:42:29ZStable transformation of erysiphe graminis an obligate biotrophic pathogen of barley.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d7a0deaf-de2e-4b18-8ca7-97cd1613eb21EnglishSymplectic Elements at Oxford2000Chaure, PGurr, SSpanu, PBarley powdery mildew, Erysiphe graminis f.sp. hordei, is an obligate biotrophic pathogen and as such cannot complete its life cycle without a living host. The inability to transform this fungus and manipulate its genome has constrained research towards understanding its life cycle and pathogenicity. Here we describe an in planta transformation system based on delivery of DNA using a gold-particle gun and selection using benomyl or bialaphos. Using this method, we consistently obtained stable transformants with efficiencies comparable to other filamentous fungi. Stable expression of the beta-glucuronidase in E. graminis was demonstrated by co-transforming the uidA gene with the selectable markers. |
| spellingShingle | Chaure, P Gurr, S Spanu, P Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley. |
| title | Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley. |
| title_full | Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley. |
| title_fullStr | Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley. |
| title_full_unstemmed | Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley. |
| title_short | Stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley. |
| title_sort | stable transformation of erysiphe graminis an obligate biotrophic pathogen of barley |
| work_keys_str_mv | AT chaurep stabletransformationoferysiphegraminisanobligatebiotrophicpathogenofbarley AT gurrs stabletransformationoferysiphegraminisanobligatebiotrophicpathogenofbarley AT spanup stabletransformationoferysiphegraminisanobligatebiotrophicpathogenofbarley |