Lack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infection
Interferon-induced transmembrane 3 (IFITM3) is known to restrict the entry of a range of enveloped viruses. The single nucleotide polymorphism rs12252-C within IFITM3 has been shown to be associated with severe influenza A virus infection. It has been suggested that rs12252-C results in expression o...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2017
|
| _version_ | 1826299417928400896 |
|---|---|
| author | Makvandi-Nejad, S Laurenson-Schafer, H Wang, L Wellington, D Zhao, Y Jin, B Qin, L Kite, K Moghadam, H Song, C Clark, K Hublitz, P Townsend, A Wu, H McMichael, A Zhang, Y Dong, T |
| author_facet | Makvandi-Nejad, S Laurenson-Schafer, H Wang, L Wellington, D Zhao, Y Jin, B Qin, L Kite, K Moghadam, H Song, C Clark, K Hublitz, P Townsend, A Wu, H McMichael, A Zhang, Y Dong, T |
| author_sort | Makvandi-Nejad, S |
| collection | OXFORD |
| description | Interferon-induced transmembrane 3 (IFITM3) is known to restrict the entry of a range of enveloped viruses. The single nucleotide polymorphism rs12252-C within IFITM3 has been shown to be associated with severe influenza A virus infection. It has been suggested that rs12252-C results in expression of a truncated IFITM3 protein lacking the first 21 amino acids. By performing high-throughput RNA sequencing on primary dendritic cells and peripheral blood mononuclear cells isolated from pandemic H1N1 influenza and human immunodeficiency virus-1 (HIV-1) infected patients we show that full-length IFITM3 mRNA is dominantly expressed (>99%) across all rs12252 genotypes. Full-length IFITM3 protein can be detected in all genotypes. |
| first_indexed | 2024-03-07T05:01:38Z |
| format | Journal article |
| id | oxford-uuid:d878e4de-eca4-4c67-a1ed-64af9624a84f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:01:38Z |
| publishDate | 2017 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:d878e4de-eca4-4c67-a1ed-64af9624a84f2022-03-27T08:48:56ZLack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infectionJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d878e4de-eca4-4c67-a1ed-64af9624a84fEnglishSymplectic Elements at OxfordOxford University Press2017Makvandi-Nejad, SLaurenson-Schafer, HWang, LWellington, DZhao, YJin, BQin, LKite, KMoghadam, HSong, CClark, KHublitz, PTownsend, AWu, HMcMichael, AZhang, YDong, TInterferon-induced transmembrane 3 (IFITM3) is known to restrict the entry of a range of enveloped viruses. The single nucleotide polymorphism rs12252-C within IFITM3 has been shown to be associated with severe influenza A virus infection. It has been suggested that rs12252-C results in expression of a truncated IFITM3 protein lacking the first 21 amino acids. By performing high-throughput RNA sequencing on primary dendritic cells and peripheral blood mononuclear cells isolated from pandemic H1N1 influenza and human immunodeficiency virus-1 (HIV-1) infected patients we show that full-length IFITM3 mRNA is dominantly expressed (>99%) across all rs12252 genotypes. Full-length IFITM3 protein can be detected in all genotypes. |
| spellingShingle | Makvandi-Nejad, S Laurenson-Schafer, H Wang, L Wellington, D Zhao, Y Jin, B Qin, L Kite, K Moghadam, H Song, C Clark, K Hublitz, P Townsend, A Wu, H McMichael, A Zhang, Y Dong, T Lack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infection |
| title | Lack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infection |
| title_full | Lack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infection |
| title_fullStr | Lack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infection |
| title_full_unstemmed | Lack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infection |
| title_short | Lack of truncated IFITM3 transcripts in cells homozygous for the rs12252-C variant that is associated with severe influenza infection |
| title_sort | lack of truncated ifitm3 transcripts in cells homozygous for the rs12252 c variant that is associated with severe influenza infection |
| work_keys_str_mv | AT makvandinejads lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT laurensonschaferh lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT wangl lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT wellingtond lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT zhaoy lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT jinb lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT qinl lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT kitek lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT moghadamh lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT songc lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT clarkk lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT hublitzp lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT townsenda lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT wuh lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT mcmichaela lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT zhangy lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection AT dongt lackoftruncatedifitm3transcriptsincellshomozygousforthers12252cvariantthatisassociatedwithsevereinfluenzainfection |