Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro
<p><strong>Aims:</strong> To investigate exosomes as a non-invasive delivery tool for mammalian sperm.</p> <p><strong>Materials & Methods:</strong> Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro.</p> <p>...
| Główni autorzy: | , , , , , , |
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| Format: | Journal article |
| Język: | English |
| Wydane: |
Future Medicine
2020
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| _version_ | 1826299466564501504 |
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| author | Coward, K Vilanova-Perez, T Jones, C Balint, S Dragovic, R Dustin, M Yeste, M |
| author_facet | Coward, K Vilanova-Perez, T Jones, C Balint, S Dragovic, R Dustin, M Yeste, M |
| author_sort | Coward, K |
| collection | OXFORD |
| description | <p><strong>Aims:</strong> To investigate exosomes as a non-invasive delivery tool for mammalian sperm.</p>
<p><strong>Materials & Methods:</strong> Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro.</p>
<p><strong>Results:</strong> Internalized exosomes were detected within 10 minutes of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-hours of exposure to exosomes, there were no significant deleterious effects with regards to sperm motility, viability, membrane integrity and mitochondrial membrane potential (P>0.05), thus indicating that exosomes did not interfere with basic sperm function.</p>
<p><strong>Conclusion:</strong> HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.</p> |
| first_indexed | 2024-03-07T05:02:21Z |
| format | Journal article |
| id | oxford-uuid:d8b93d3c-4e3a-4d24-abb5-f9f9b017c722 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:02:21Z |
| publishDate | 2020 |
| publisher | Future Medicine |
| record_format | dspace |
| spelling | oxford-uuid:d8b93d3c-4e3a-4d24-abb5-f9f9b017c7222022-03-27T08:50:51ZExosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitroJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d8b93d3c-4e3a-4d24-abb5-f9f9b017c722EnglishSymplectic ElementsFuture Medicine2020Coward, KVilanova-Perez, TJones, CBalint, SDragovic, RDustin, MYeste, M<p><strong>Aims:</strong> To investigate exosomes as a non-invasive delivery tool for mammalian sperm.</p> <p><strong>Materials & Methods:</strong> Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro.</p> <p><strong>Results:</strong> Internalized exosomes were detected within 10 minutes of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-hours of exposure to exosomes, there were no significant deleterious effects with regards to sperm motility, viability, membrane integrity and mitochondrial membrane potential (P>0.05), thus indicating that exosomes did not interfere with basic sperm function.</p> <p><strong>Conclusion:</strong> HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.</p> |
| spellingShingle | Coward, K Vilanova-Perez, T Jones, C Balint, S Dragovic, R Dustin, M Yeste, M Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro |
| title | Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro |
| title_full | Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro |
| title_fullStr | Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro |
| title_full_unstemmed | Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro |
| title_short | Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro |
| title_sort | exosomes derived from hek293t cells interact in an efficient and non invasive manner with mammalian sperm in vitro |
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