Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats.
The Bunyavirus genus, belonging to the Bunyaviridae family, is comprised of a large group of antigenically and geographically disparate arthropod-borne viruses of medical and veterinary significance. In Australia, viruses belonging to the Simbu serogroup of the Bunyavirus genus, Akabane, Tinaroo, Pe...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1997
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| _version_ | 1826299502933311488 |
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| author | Blacksell, S Lunt, R White, JR |
| author_facet | Blacksell, S Lunt, R White, JR |
| author_sort | Blacksell, S |
| collection | OXFORD |
| description | The Bunyavirus genus, belonging to the Bunyaviridae family, is comprised of a large group of antigenically and geographically disparate arthropod-borne viruses of medical and veterinary significance. In Australia, viruses belonging to the Simbu serogroup of the Bunyavirus genus, Akabane, Tinaroo, Peaton, Aino, Douglas, Thimiri and Facey's Paddock have been isolated. In this communication we describe two indirect ELISAs, referred to as the Simbu serogroup ELISA (SG-ELISA), and the Simbu typing ELISA (ST-ELISA), for the identification of these Simbu serogroup viruses. Infected cell lysate antigens prepared from Simbu serogroup virus isolates were assessed in the SG-ELISA for reactivity with a mouse monoclonal antibody (4H9/B11/F1). The monoclonal antibody reacted strongly with all Australian members of Simbu serogroup reference viruses and is proposed for use as a serogrouping reagent for Simbu viruses. Furthermore, the ST-ELISA enabled specific identification of viruses from within this group by recognition of characteristic reaction patterns between infected cell lysate antigens and a panel of polyclonal antisera raised to Simbu serogroup viruses. |
| first_indexed | 2024-03-07T05:02:55Z |
| format | Journal article |
| id | oxford-uuid:d8edf51e-3306-4f1a-b7a4-7e66129a4e83 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:02:55Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:d8edf51e-3306-4f1a-b7a4-7e66129a4e832022-03-27T08:52:19ZRapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d8edf51e-3306-4f1a-b7a4-7e66129a4e83EnglishSymplectic Elements at Oxford1997Blacksell, SLunt, RWhite, JRThe Bunyavirus genus, belonging to the Bunyaviridae family, is comprised of a large group of antigenically and geographically disparate arthropod-borne viruses of medical and veterinary significance. In Australia, viruses belonging to the Simbu serogroup of the Bunyavirus genus, Akabane, Tinaroo, Peaton, Aino, Douglas, Thimiri and Facey's Paddock have been isolated. In this communication we describe two indirect ELISAs, referred to as the Simbu serogroup ELISA (SG-ELISA), and the Simbu typing ELISA (ST-ELISA), for the identification of these Simbu serogroup viruses. Infected cell lysate antigens prepared from Simbu serogroup virus isolates were assessed in the SG-ELISA for reactivity with a mouse monoclonal antibody (4H9/B11/F1). The monoclonal antibody reacted strongly with all Australian members of Simbu serogroup reference viruses and is proposed for use as a serogrouping reagent for Simbu viruses. Furthermore, the ST-ELISA enabled specific identification of viruses from within this group by recognition of characteristic reaction patterns between infected cell lysate antigens and a panel of polyclonal antisera raised to Simbu serogroup viruses. |
| spellingShingle | Blacksell, S Lunt, R White, JR Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats. |
| title | Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats. |
| title_full | Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats. |
| title_fullStr | Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats. |
| title_full_unstemmed | Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats. |
| title_short | Rapid identification of Australian bunyavirus isolates belonging to the Simbu serogroup using indirect ELISA formats. |
| title_sort | rapid identification of australian bunyavirus isolates belonging to the simbu serogroup using indirect elisa formats |
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