Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates.
Platelet-endothelial cell adhesion molecule-1 or CD31 (PECAM-1/CD31) is a receptor recognized by Plasmodium falciparum-parasitized erythrocytes (pRBCs). Fluorescence-labeled soluble recombinant PECAM-1/CD31 (sPECAM-1/CD31) is shown to bind to the surface of P. falciparum-infected erythrocytes on up...
| Glavni autori: | , , , , , , , |
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| Format: | Journal article |
| Jezik: | English |
| Izdano: |
2001
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| _version_ | 1826299509059092480 |
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| author | Heddini, A Chen, Q Obiero, J Kai, O Fernandez, V Marsh, K Muller, W Wahlgren, M |
| author_facet | Heddini, A Chen, Q Obiero, J Kai, O Fernandez, V Marsh, K Muller, W Wahlgren, M |
| author_sort | Heddini, A |
| collection | OXFORD |
| description | Platelet-endothelial cell adhesion molecule-1 or CD31 (PECAM-1/CD31) is a receptor recognized by Plasmodium falciparum-parasitized erythrocytes (pRBCs). Fluorescence-labeled soluble recombinant PECAM-1/CD31 (sPECAM-1/CD31) is shown to bind to the surface of P. falciparum-infected erythrocytes on up to 70% of the cells. Binding is blocked by the addition of the unlabeled receptor in a dose-dependent fashion, but not by unrelated receptor-proteins. A significant correlation was found between the binding of sPECAM-1/CD31 to pRBCs and the binding to transfected L cells expressing the receptor as seen with six different P. falciparum lines or clones. Panning of cultures on PECAM-1/CD31 transfected L cells was paralleled by an increase in the binding of sPECAM-1/CD31. The pRBCs of 54% of fresh patient-isolates bound sPECAM-1/CD31 with a mean rate of 12.9% (range = 1.1-44%). The data suggest that PECAM-1/CD31 is a common receptor recognized by wild isolates and that the soluble PECAM-1/CD31 suspension assay is a sensitive and reliable way to study PECAM-1/CD31 binding. |
| first_indexed | 2024-03-07T05:03:01Z |
| format | Journal article |
| id | oxford-uuid:d8f57dd4-8ce3-4602-a1cf-75d3f3d90a7d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:03:01Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:d8f57dd4-8ce3-4602-a1cf-75d3f3d90a7d2022-03-27T08:52:30ZBinding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d8f57dd4-8ce3-4602-a1cf-75d3f3d90a7dEnglishSymplectic Elements at Oxford2001Heddini, AChen, QObiero, JKai, OFernandez, VMarsh, KMuller, WWahlgren, MPlatelet-endothelial cell adhesion molecule-1 or CD31 (PECAM-1/CD31) is a receptor recognized by Plasmodium falciparum-parasitized erythrocytes (pRBCs). Fluorescence-labeled soluble recombinant PECAM-1/CD31 (sPECAM-1/CD31) is shown to bind to the surface of P. falciparum-infected erythrocytes on up to 70% of the cells. Binding is blocked by the addition of the unlabeled receptor in a dose-dependent fashion, but not by unrelated receptor-proteins. A significant correlation was found between the binding of sPECAM-1/CD31 to pRBCs and the binding to transfected L cells expressing the receptor as seen with six different P. falciparum lines or clones. Panning of cultures on PECAM-1/CD31 transfected L cells was paralleled by an increase in the binding of sPECAM-1/CD31. The pRBCs of 54% of fresh patient-isolates bound sPECAM-1/CD31 with a mean rate of 12.9% (range = 1.1-44%). The data suggest that PECAM-1/CD31 is a common receptor recognized by wild isolates and that the soluble PECAM-1/CD31 suspension assay is a sensitive and reliable way to study PECAM-1/CD31 binding. |
| spellingShingle | Heddini, A Chen, Q Obiero, J Kai, O Fernandez, V Marsh, K Muller, W Wahlgren, M Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates. |
| title | Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates. |
| title_full | Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates. |
| title_fullStr | Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates. |
| title_full_unstemmed | Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates. |
| title_short | Binding of Plasmodium falciparum-infected erythrocytes to soluble platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): frequent recognition by clinical isolates. |
| title_sort | binding of plasmodium falciparum infected erythrocytes to soluble platelet endothelial cell adhesion molecule 1 pecam 1 cd31 frequent recognition by clinical isolates |
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