Regulation of pulmonary plasma cell responses during secondary infection with influenza virus
During secondary infection with influenza virus, plasma cells (PCs) develop within the lung, providing a local source of antibodies. However, the site and mechanisms that regulate this process are poorly defined. Here, we show that while circulating memory B cells entered the lung during rechallenge...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Rockefeller University Press
2024
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| _version_ | 1811139658456760320 |
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| author | Maclean, AJ Bonifacio Lopes, J Oram, S Mohsen, M Bachmann, M Arnon, TI |
| author_facet | Maclean, AJ Bonifacio Lopes, J Oram, S Mohsen, M Bachmann, M Arnon, TI |
| author_sort | Maclean, AJ |
| collection | OXFORD |
| description | During secondary infection with influenza virus, plasma cells (PCs) develop within the lung, providing a local source of antibodies. However, the site and mechanisms that regulate this process are poorly defined. Here, we show that while circulating memory B cells entered the lung during rechallenge and were activated within inducible bronchus-associated lymphoid tissues (iBALTs), resident memory B (BRM) cells responded earlier, and their activation occurred in a different niche: directly near infected alveoli. This process required NK cells but was largely independent of CD4 and CD8 T cells. Innate stimuli induced by virus-like particles containing ssRNA triggered BRM cell differentiation in the absence of cognate antigen, suggesting a low threshold of activation. In contrast, expansion of PCs in iBALTs took longer to develop and was critically dependent on CD4 T cells. Our work demonstrates that spatially distinct mechanisms evolved to support pulmonary secondary PC responses, and it reveals a specialized function for BRM cells as guardians of the alveoli. |
| first_indexed | 2024-04-23T08:26:28Z |
| format | Journal article |
| id | oxford-uuid:d90792b3-e2fd-4ae6-b53b-5c72ef66ab22 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:09:35Z |
| publishDate | 2024 |
| publisher | Rockefeller University Press |
| record_format | dspace |
| spelling | oxford-uuid:d90792b3-e2fd-4ae6-b53b-5c72ef66ab222024-06-07T11:21:59ZRegulation of pulmonary plasma cell responses during secondary infection with influenza virusJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d90792b3-e2fd-4ae6-b53b-5c72ef66ab22EnglishSymplectic ElementsRockefeller University Press2024Maclean, AJBonifacio Lopes, JOram, SMohsen, MBachmann, MArnon, TIDuring secondary infection with influenza virus, plasma cells (PCs) develop within the lung, providing a local source of antibodies. However, the site and mechanisms that regulate this process are poorly defined. Here, we show that while circulating memory B cells entered the lung during rechallenge and were activated within inducible bronchus-associated lymphoid tissues (iBALTs), resident memory B (BRM) cells responded earlier, and their activation occurred in a different niche: directly near infected alveoli. This process required NK cells but was largely independent of CD4 and CD8 T cells. Innate stimuli induced by virus-like particles containing ssRNA triggered BRM cell differentiation in the absence of cognate antigen, suggesting a low threshold of activation. In contrast, expansion of PCs in iBALTs took longer to develop and was critically dependent on CD4 T cells. Our work demonstrates that spatially distinct mechanisms evolved to support pulmonary secondary PC responses, and it reveals a specialized function for BRM cells as guardians of the alveoli. |
| spellingShingle | Maclean, AJ Bonifacio Lopes, J Oram, S Mohsen, M Bachmann, M Arnon, TI Regulation of pulmonary plasma cell responses during secondary infection with influenza virus |
| title | Regulation of pulmonary plasma cell responses during secondary infection with influenza virus |
| title_full | Regulation of pulmonary plasma cell responses during secondary infection with influenza virus |
| title_fullStr | Regulation of pulmonary plasma cell responses during secondary infection with influenza virus |
| title_full_unstemmed | Regulation of pulmonary plasma cell responses during secondary infection with influenza virus |
| title_short | Regulation of pulmonary plasma cell responses during secondary infection with influenza virus |
| title_sort | regulation of pulmonary plasma cell responses during secondary infection with influenza virus |
| work_keys_str_mv | AT macleanaj regulationofpulmonaryplasmacellresponsesduringsecondaryinfectionwithinfluenzavirus AT bonifaciolopesj regulationofpulmonaryplasmacellresponsesduringsecondaryinfectionwithinfluenzavirus AT orams regulationofpulmonaryplasmacellresponsesduringsecondaryinfectionwithinfluenzavirus AT mohsenm regulationofpulmonaryplasmacellresponsesduringsecondaryinfectionwithinfluenzavirus AT bachmannm regulationofpulmonaryplasmacellresponsesduringsecondaryinfectionwithinfluenzavirus AT arnonti regulationofpulmonaryplasmacellresponsesduringsecondaryinfectionwithinfluenzavirus |