Splicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein.
RNA segment 8 (NS) of influenza A virus encodes two proteins. The NS1 protein is translated from the unspliced primary mRNA transcript, whereas the second protein encoded by this segment, NS2/NEP, is translated from a spliced mRNA. Splicing of influenza NS1 mRNA is thought to be regulated so that th...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2010
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| _version_ | 1797098000975134720 |
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| author | Robb, N Jackson, D Vreede, F Fodor, E |
| author_facet | Robb, N Jackson, D Vreede, F Fodor, E |
| author_sort | Robb, N |
| collection | OXFORD |
| description | RNA segment 8 (NS) of influenza A virus encodes two proteins. The NS1 protein is translated from the unspliced primary mRNA transcript, whereas the second protein encoded by this segment, NS2/NEP, is translated from a spliced mRNA. Splicing of influenza NS1 mRNA is thought to be regulated so that the levels of NS2 spliced transcripts are approximately 10 % of total NS mRNA. Regulation of splicing of the NS1 mRNA has been studied at length, and a number of often-contradictory control mechanisms have been proposed. In this study, we used (32)P-labelled gene-specific primers to investigate influenza A NS1 mRNA splicing regulation. It was found that the efficiency of splicing of NS1 mRNA was maintained at similar levels in both virus infection and ribonucleoprotein-reconstitution assays, and NS2 mRNA comprised approximately 15 % of total NS mRNA in both assays. The effect of NS1 protein expression on the accumulation of viral NS2 mRNA and spliced cellular beta-globin mRNA was analysed, and it was found that NS1 protein expression reduced spliced beta-globin mRNA levels, but had no effect on the accumulation of NS2 mRNA. We conclude that the NS1 protein specifically inhibits the accumulation of cellular RNA polymerase II-driven mRNAs, but does not affect the splicing of its own viral NS1 mRNA. |
| first_indexed | 2024-03-07T05:03:23Z |
| format | Journal article |
| id | oxford-uuid:d9185257-5626-4f48-8e14-2550b1d53faf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:03:23Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:d9185257-5626-4f48-8e14-2550b1d53faf2022-03-27T08:53:23ZSplicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d9185257-5626-4f48-8e14-2550b1d53fafEnglishSymplectic Elements at Oxford2010Robb, NJackson, DVreede, FFodor, ERNA segment 8 (NS) of influenza A virus encodes two proteins. The NS1 protein is translated from the unspliced primary mRNA transcript, whereas the second protein encoded by this segment, NS2/NEP, is translated from a spliced mRNA. Splicing of influenza NS1 mRNA is thought to be regulated so that the levels of NS2 spliced transcripts are approximately 10 % of total NS mRNA. Regulation of splicing of the NS1 mRNA has been studied at length, and a number of often-contradictory control mechanisms have been proposed. In this study, we used (32)P-labelled gene-specific primers to investigate influenza A NS1 mRNA splicing regulation. It was found that the efficiency of splicing of NS1 mRNA was maintained at similar levels in both virus infection and ribonucleoprotein-reconstitution assays, and NS2 mRNA comprised approximately 15 % of total NS mRNA in both assays. The effect of NS1 protein expression on the accumulation of viral NS2 mRNA and spliced cellular beta-globin mRNA was analysed, and it was found that NS1 protein expression reduced spliced beta-globin mRNA levels, but had no effect on the accumulation of NS2 mRNA. We conclude that the NS1 protein specifically inhibits the accumulation of cellular RNA polymerase II-driven mRNAs, but does not affect the splicing of its own viral NS1 mRNA. |
| spellingShingle | Robb, N Jackson, D Vreede, F Fodor, E Splicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein. |
| title | Splicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein. |
| title_full | Splicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein. |
| title_fullStr | Splicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein. |
| title_full_unstemmed | Splicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein. |
| title_short | Splicing of influenza A virus NS1 mRNA is independent of the viral NS1 protein. |
| title_sort | splicing of influenza a virus ns1 mrna is independent of the viral ns1 protein |
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