Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity.
Reports from a number of laboratories have shown that mAbs against the T3-Ti receptor complex cause an increase in cytosolic-free Ca2+ [( Ca2+]i) and the hydrolysis of phosphatidylinositolbisphosphate (PIP2) in CTLs. In the present report we show that activation of CTLs by their specific targets cau...
| Autori principali: | , , , , , |
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| Natura: | Journal article |
| Lingua: | English |
| Pubblicazione: |
1987
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| _version_ | 1826299575351115776 |
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| author | Treves, S Di Virgilio, F Cerundolo, V Zanovello, P Collavo, D Pozzan, T |
| author_facet | Treves, S Di Virgilio, F Cerundolo, V Zanovello, P Collavo, D Pozzan, T |
| author_sort | Treves, S |
| collection | OXFORD |
| description | Reports from a number of laboratories have shown that mAbs against the T3-Ti receptor complex cause an increase in cytosolic-free Ca2+ [( Ca2+]i) and the hydrolysis of phosphatidylinositolbisphosphate (PIP2) in CTLs. In the present report we show that activation of CTLs by their specific targets causes: (a) release of Ca2+ from intracellular stores; (b) transient formation of inositol trisphosphate (InsP3); and (c) an increased permeability to Ca2+ of CTL plasma membrane. Killing of unrelated targets could be induced by cocentrifugation of the unrelated targets with CTLs in the presence of A23187 or PMA. We conclude that: (a) activation of CTLs by specific antigens triggers the generation of the same intracellular mediators generated by stimulation of lymphocytes with anti-T3-Ti receptor antibodies and/or with polyclonal mitogens; and (b) intracellular signals that mediate the delivery of the lethal hit by CTLs are indistinguishable from those that induce cell proliferation. |
| first_indexed | 2024-03-07T05:04:01Z |
| format | Journal article |
| id | oxford-uuid:d94a8f18-13c1-48e9-9df9-79edf8909eac |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:04:01Z |
| publishDate | 1987 |
| record_format | dspace |
| spelling | oxford-uuid:d94a8f18-13c1-48e9-9df9-79edf8909eac2022-03-27T08:54:52ZCalcium and inositolphosphates in the activation of T cell-mediated cytotoxicity.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d94a8f18-13c1-48e9-9df9-79edf8909eacEnglishSymplectic Elements at Oxford1987Treves, SDi Virgilio, FCerundolo, VZanovello, PCollavo, DPozzan, TReports from a number of laboratories have shown that mAbs against the T3-Ti receptor complex cause an increase in cytosolic-free Ca2+ [( Ca2+]i) and the hydrolysis of phosphatidylinositolbisphosphate (PIP2) in CTLs. In the present report we show that activation of CTLs by their specific targets causes: (a) release of Ca2+ from intracellular stores; (b) transient formation of inositol trisphosphate (InsP3); and (c) an increased permeability to Ca2+ of CTL plasma membrane. Killing of unrelated targets could be induced by cocentrifugation of the unrelated targets with CTLs in the presence of A23187 or PMA. We conclude that: (a) activation of CTLs by specific antigens triggers the generation of the same intracellular mediators generated by stimulation of lymphocytes with anti-T3-Ti receptor antibodies and/or with polyclonal mitogens; and (b) intracellular signals that mediate the delivery of the lethal hit by CTLs are indistinguishable from those that induce cell proliferation. |
| spellingShingle | Treves, S Di Virgilio, F Cerundolo, V Zanovello, P Collavo, D Pozzan, T Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity. |
| title | Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity. |
| title_full | Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity. |
| title_fullStr | Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity. |
| title_full_unstemmed | Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity. |
| title_short | Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity. |
| title_sort | calcium and inositolphosphates in the activation of t cell mediated cytotoxicity |
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