A compact monomeric intermediate identified by NMR in the denaturation of dimeric triose phosphate isomerase.

A compact monomeric intermediate identified by NMR in the denaturation of dimeric triose phosphate isomerase.

The denaturation of triose phosphate isomerase (TIM) from Saccharomyces cerevisiae by guanidine hydrochlorids at pH 7.2 has been monitored by NMR spectroscopy in conjunction with optical spectroscopy. In the absence of denaturant, the hydrodynamic radius of 29.6(+/-0.25) A and the substantial chemic...

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Bibliographic Details
Main Authors:	Morgan, C, Wilkins, D, Smith, L, Kawata, Y, Dobson, C
Format:	Journal article
Language:	English
Published:	2000

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