Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly.
Synapse formation and maturation requires bidirectional communication across the synaptic cleft. The trans-synaptic Neurexin-Neuroligin complex can bridge this cleft, and severe synapse assembly deficits are found in Drosophila melanogaster neuroligin (Nlg1, dnlg1) and neurexin (Nrx-1, dnrx) mutants...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1797098163230736384 |
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| author | Owald, D Khorramshahi, O Gupta, V Banovic, D Depner, H Fouquet, W Wichmann, C Mertel, S Eimer, S Reynolds, E Holt, M Aberle, H Sigrist, S |
| author_facet | Owald, D Khorramshahi, O Gupta, V Banovic, D Depner, H Fouquet, W Wichmann, C Mertel, S Eimer, S Reynolds, E Holt, M Aberle, H Sigrist, S |
| author_sort | Owald, D |
| collection | OXFORD |
| description | Synapse formation and maturation requires bidirectional communication across the synaptic cleft. The trans-synaptic Neurexin-Neuroligin complex can bridge this cleft, and severe synapse assembly deficits are found in Drosophila melanogaster neuroligin (Nlg1, dnlg1) and neurexin (Nrx-1, dnrx) mutants. We show that the presynaptic active zone protein Syd-1 interacts with Nrx-1 to control synapse formation at the Drosophila neuromuscular junction. Mutants in Syd-1 (RhoGAP100F, dsyd-1), Nrx-1 and Nlg1 shared active zone cytomatrix defects, which were nonadditive. Syd-1 and Nrx-1 formed a complex in vivo, and Syd-1 was important for synaptic clustering and immobilization of Nrx-1. Consequently, postsynaptic clustering of Nlg1 was affected in Syd-1 mutants, and in vivo glutamate receptor incorporation was changed in Syd-1, Nrx-1 and Nlg1 mutants. Stabilization of nascent Syd-1-Liprin-α (DLiprin-α) clusters, important to initialize active zone formation, was Nlg1 dependent. Thus, cooperation between Syd-1 and Nrx-1-Nlg1 seems to orchestrate early assembly processes between pre- and postsynaptic membranes, promoting avidity of newly forming synaptic scaffolds. |
| first_indexed | 2024-03-07T05:05:40Z |
| format | Journal article |
| id | oxford-uuid:d9d24f00-fdaf-44f2-95e7-4243e1b6f50a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:05:40Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:d9d24f00-fdaf-44f2-95e7-4243e1b6f50a2022-03-27T08:58:45ZCooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:d9d24f00-fdaf-44f2-95e7-4243e1b6f50aEnglishSymplectic Elements at Oxford2012Owald, DKhorramshahi, OGupta, VBanovic, DDepner, HFouquet, WWichmann, CMertel, SEimer, SReynolds, EHolt, MAberle, HSigrist, SSynapse formation and maturation requires bidirectional communication across the synaptic cleft. The trans-synaptic Neurexin-Neuroligin complex can bridge this cleft, and severe synapse assembly deficits are found in Drosophila melanogaster neuroligin (Nlg1, dnlg1) and neurexin (Nrx-1, dnrx) mutants. We show that the presynaptic active zone protein Syd-1 interacts with Nrx-1 to control synapse formation at the Drosophila neuromuscular junction. Mutants in Syd-1 (RhoGAP100F, dsyd-1), Nrx-1 and Nlg1 shared active zone cytomatrix defects, which were nonadditive. Syd-1 and Nrx-1 formed a complex in vivo, and Syd-1 was important for synaptic clustering and immobilization of Nrx-1. Consequently, postsynaptic clustering of Nlg1 was affected in Syd-1 mutants, and in vivo glutamate receptor incorporation was changed in Syd-1, Nrx-1 and Nlg1 mutants. Stabilization of nascent Syd-1-Liprin-α (DLiprin-α) clusters, important to initialize active zone formation, was Nlg1 dependent. Thus, cooperation between Syd-1 and Nrx-1-Nlg1 seems to orchestrate early assembly processes between pre- and postsynaptic membranes, promoting avidity of newly forming synaptic scaffolds. |
| spellingShingle | Owald, D Khorramshahi, O Gupta, V Banovic, D Depner, H Fouquet, W Wichmann, C Mertel, S Eimer, S Reynolds, E Holt, M Aberle, H Sigrist, S Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly. |
| title | Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly. |
| title_full | Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly. |
| title_fullStr | Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly. |
| title_full_unstemmed | Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly. |
| title_short | Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly. |
| title_sort | cooperation of syd 1 with neurexin synchronizes pre with postsynaptic assembly |
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