MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme

Efficient DNA assembly is of great value in biological research and biotechnology. Type IIS restriction enzyme-based assembly systems allow assembly of multiple DNA fragments in a one-pot reaction. However, large DNA fragments can only be assembled by alternating use of two or more type IIS restrict...

Mô tả đầy đủ

Chi tiết về thư mục
Những tác giả chính: Lin, D, O'Callaghan, C
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: Oxford University Press 2018