Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors.
Cyclic AMP governs many fundamental signaling events in eukaryotic cells. Although cAMP signaling has been a major research focus for a long time, recent technological developments are revealing novel aspects of this paradigmatic pathway. In this chapter, we give an overview over current fluorescenc...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2011
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| _version_ | 1797098215196065792 |
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| author | Gesellchen, F Stangherlin, A Surdo, N Terrin, A Zoccarato, A Zaccolo, M |
| author_facet | Gesellchen, F Stangherlin, A Surdo, N Terrin, A Zoccarato, A Zaccolo, M |
| author_sort | Gesellchen, F |
| collection | OXFORD |
| description | Cyclic AMP governs many fundamental signaling events in eukaryotic cells. Although cAMP signaling has been a major research focus for a long time, recent technological developments are revealing novel aspects of this paradigmatic pathway. In this chapter, we give an overview over current fluorescence resonance energy transfer (FRET)-based sensors for detection of cAMP dynamics, and their application in monitoring local, compartmentalized cAMP signals within living cells. A basic step-by-step protocol is given for conducting a FRET experiment in primary cells with a unimolecular cAMP sensor, which can easily be adapted to a user's specific requirements. |
| first_indexed | 2024-03-07T05:06:26Z |
| format | Journal article |
| id | oxford-uuid:da13eb5f-cf6b-45ea-baf1-c9fd50c1bba9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:06:26Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oxford-uuid:da13eb5f-cf6b-45ea-baf1-c9fd50c1bba92022-03-27T09:00:34ZMeasuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:da13eb5f-cf6b-45ea-baf1-c9fd50c1bba9EnglishSymplectic Elements at Oxford2011Gesellchen, FStangherlin, ASurdo, NTerrin, AZoccarato, AZaccolo, MCyclic AMP governs many fundamental signaling events in eukaryotic cells. Although cAMP signaling has been a major research focus for a long time, recent technological developments are revealing novel aspects of this paradigmatic pathway. In this chapter, we give an overview over current fluorescence resonance energy transfer (FRET)-based sensors for detection of cAMP dynamics, and their application in monitoring local, compartmentalized cAMP signals within living cells. A basic step-by-step protocol is given for conducting a FRET experiment in primary cells with a unimolecular cAMP sensor, which can easily be adapted to a user's specific requirements. |
| spellingShingle | Gesellchen, F Stangherlin, A Surdo, N Terrin, A Zoccarato, A Zaccolo, M Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. |
| title | Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. |
| title_full | Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. |
| title_fullStr | Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. |
| title_full_unstemmed | Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. |
| title_short | Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors. |
| title_sort | measuring spatiotemporal dynamics of cyclic amp signaling in real time using fret based biosensors |
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