Visualization and measurement of DNA methyltransferase activity in living cells.

In this unit, a live-cell assay to measure DNA (cytosine-5) methyltransferase (MTase) activity at the single-cell level is described. This method takes advantage of the irreversible binding of enzymatically active MTases to genomic DNA substituted with the mechanism-based inhibitor 5-aza-2'-deo...

Mô tả đầy đủ

Chi tiết về thư mục
Những tác giả chính: Schermelleh, L, Spada, F, Leonhardt, H
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: 2008