Intravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response.
Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1(157-165)-specific CD8(+) cells...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1826299904093323264 |
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| author | Palmowski, M Lopes, L Ikeda, Y Salio, M Cerundolo, V Collins, M |
| author_facet | Palmowski, M Lopes, L Ikeda, Y Salio, M Cerundolo, V Collins, M |
| author_sort | Palmowski, M |
| collection | OXFORD |
| description | Lentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1(157-165)-specific CD8(+) cells, detected ex vivo with an A2/H-2K(b) chimeric class I tetramer. These NY-ESO-1(157-165)-specific CD8(+) cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1(157-165) peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1(157-165)-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy. |
| first_indexed | 2024-03-07T05:09:01Z |
| format | Journal article |
| id | oxford-uuid:daecc26a-e031-4cff-b04f-da3478ee23a8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:09:01Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:daecc26a-e031-4cff-b04f-da3478ee23a82022-03-27T09:06:40ZIntravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:daecc26a-e031-4cff-b04f-da3478ee23a8EnglishSymplectic Elements at Oxford2004Palmowski, MLopes, LIkeda, YSalio, MCerundolo, VCollins, MLentiviral vectors can efficiently transduce a variety of nondividing cells, including APCs. We assessed the immunogenicity of a lentiviral vector encoding the melanoma Ag NY-ESO-1 in HLA-A2 transgenic mice. Direct i.v. injection of NY-ESO-1 lentivirus induced NY-ESO-1(157-165)-specific CD8(+) cells, detected ex vivo with an A2/H-2K(b) chimeric class I tetramer. These NY-ESO-1(157-165)-specific CD8(+) cells could be expanded by boosting with an NY-ESO-1 vaccinia virus and could kill NY-ESO-1(157-165) peptide-pulsed targets in vivo. Such direct lentiviral vector injection was similar in potency to the injection of in vitro-transduced dendritic cells (DC). In addition, human monocyte-derived DC transduced by the NY-ESO-1 lentivirus stimulated an NY-ESO-1(157-165)-specific specific CTL clone. These data suggest that direct lentiviral transduction of DC in vivo might provide a powerful immunotherapeutic strategy. |
| spellingShingle | Palmowski, M Lopes, L Ikeda, Y Salio, M Cerundolo, V Collins, M Intravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response. |
| title | Intravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response. |
| title_full | Intravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response. |
| title_fullStr | Intravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response. |
| title_full_unstemmed | Intravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response. |
| title_short | Intravenous injection of a lentiviral vector encoding NY-ESO-1 induces an effective CTL response. |
| title_sort | intravenous injection of a lentiviral vector encoding ny eso 1 induces an effective ctl response |
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