Monoclonal antibody BRL 12 reacts with bone keratan sulphate proteoglycan.

Our previous studies suggest that a monoclonal antibody, BRL 12, reacts with a specific product of differentiated cells of the osteoblastic lineage. In the present study, the bone constituent recognized by this antibody has been characterized by gel filtration, ion exchange chromatography, protein b...

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Bibliographic Details
Main Authors: Joyner, C, Virdi, A, Beresford, J, Bennett, J, Owen, M, Triffitt, J
Format: Journal article
Language:English
Published: Informa Healthcare 1992
Description
Summary:Our previous studies suggest that a monoclonal antibody, BRL 12, reacts with a specific product of differentiated cells of the osteoblastic lineage. In the present study, the bone constituent recognized by this antibody has been characterized by gel filtration, ion exchange chromatography, protein blotting and immunolocalization. Our findings show that the antibody reacts with an epitope associated with the core protein of rabbit keratan sulfate proteoglycan (KSPG), a molecule which shares considerable homology with the sialoprotein present in the bone tissue of other mammalian species.