Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction.
Myofibroblasts play a major role in scar formation during wound healing after myocardial infarction (MI). Their origin has been thought to be interstitial cardiac fibroblasts. However, the bone marrow (BM) can be a source of myofibroblasts in a number of organs after injury. We have studied the temp...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826300043377770496 |
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| author | van Amerongen, M Bou-Gharios, G Popa, E van Ark, J Petersen, A Dam, v van Luyn, M Harmsen, M |
| author_facet | van Amerongen, M Bou-Gharios, G Popa, E van Ark, J Petersen, A Dam, v van Luyn, M Harmsen, M |
| author_sort | van Amerongen, M |
| collection | OXFORD |
| description | Myofibroblasts play a major role in scar formation during wound healing after myocardial infarction (MI). Their origin has been thought to be interstitial cardiac fibroblasts. However, the bone marrow (BM) can be a source of myofibroblasts in a number of organs after injury. We have studied the temporal, quantitative and functional role of BM-derived (BMD) myofibroblasts in myocardial scar formation. MI was induced by permanent coronary artery ligation in mice reconstituted with EGFP or pro-Col1A2 transgenic BM. In the latter, luciferase and beta-galactosidase transgene expression mirrors that of the endogenous pro-collagen 1A2 gene, which allows for functional assessment of the recruited cells. After MI, alpha-SMA-positive myofibroblasts and collagen I gradually increased in the infarct area until day 14 and remained constant afterwards. Numerous EGFP-positive BMD cells were present during the first week post-MI, and gradually decreased afterwards until day 28. Peak numbers of BMD myofibroblasts, co-expressing EGFP and alpha-SMA, were found on day 7 post-MI. An average of 21% of the BMD cells in the infarct area were myofibroblasts. These cells constituted up to 24% of all myofibroblasts present. By in vivo IVIS imaging, BMD myofibroblasts were found to be active for collagen I production and their presence was confined to the infarct area. These results show that BMD myofibroblasts participate actively in scar formation after MI. |
| first_indexed | 2024-03-07T05:11:07Z |
| format | Journal article |
| id | oxford-uuid:db96992f-b389-454e-a0c5-7311447c0763 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:11:07Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:db96992f-b389-454e-a0c5-7311447c07632022-03-27T09:11:39ZBone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:db96992f-b389-454e-a0c5-7311447c0763EnglishSymplectic Elements at Oxford2008van Amerongen, MBou-Gharios, GPopa, Evan Ark, JPetersen, ADam, vvan Luyn, MHarmsen, MMyofibroblasts play a major role in scar formation during wound healing after myocardial infarction (MI). Their origin has been thought to be interstitial cardiac fibroblasts. However, the bone marrow (BM) can be a source of myofibroblasts in a number of organs after injury. We have studied the temporal, quantitative and functional role of BM-derived (BMD) myofibroblasts in myocardial scar formation. MI was induced by permanent coronary artery ligation in mice reconstituted with EGFP or pro-Col1A2 transgenic BM. In the latter, luciferase and beta-galactosidase transgene expression mirrors that of the endogenous pro-collagen 1A2 gene, which allows for functional assessment of the recruited cells. After MI, alpha-SMA-positive myofibroblasts and collagen I gradually increased in the infarct area until day 14 and remained constant afterwards. Numerous EGFP-positive BMD cells were present during the first week post-MI, and gradually decreased afterwards until day 28. Peak numbers of BMD myofibroblasts, co-expressing EGFP and alpha-SMA, were found on day 7 post-MI. An average of 21% of the BMD cells in the infarct area were myofibroblasts. These cells constituted up to 24% of all myofibroblasts present. By in vivo IVIS imaging, BMD myofibroblasts were found to be active for collagen I production and their presence was confined to the infarct area. These results show that BMD myofibroblasts participate actively in scar formation after MI. |
| spellingShingle | van Amerongen, M Bou-Gharios, G Popa, E van Ark, J Petersen, A Dam, v van Luyn, M Harmsen, M Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. |
| title | Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. |
| title_full | Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. |
| title_fullStr | Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. |
| title_full_unstemmed | Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. |
| title_short | Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. |
| title_sort | bone marrow derived myofibroblasts contribute functionally to scar formation after myocardial infarction |
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