Cell-free transcription and translation of Na,K-ATPase alpha and beta subunit cDNAs.

Cell-free transcription and translation of Na,K-ATPase alpha and beta subunit cDNAs.

Synthetic mRNAs (i.e. cRNA alpha and cRNA beta) were obtained by cell-free transcription of M13 KS(+) (Bluescript) expression vectors which contained the entire coding region of the alpha or beta subunits of lamb kidney Na,K-ATPase. Translation in reticulocyte lysates of cRNA alpha yielded full leng...

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Bibliographic Details
Main Authors:	Cayanis, E, Bayley, H, Edelman, I
Format:	Journal article
Language:	English
Published:	1990

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