A recombinant vaccinia virus based ELISPOT assay detects high frequencies of Pol-specific CD8 T cells in HIV-1-positive individuals.

A recombinant vaccinia virus based ELISPOT assay detects high frequencies of Pol-specific CD8 T cells in HIV-1-positive individuals.

OBJECTIVES: HIV-1-specific CD8 T cells are considered to be critical in anti-HIV responses. It is important to quantify these cells and to determine their antigenic targets. Here quantification of interferon (IFN)-gamma secreting, virus-specific cells was achieved with an enzyme linked immuno spot (...

Full description

Bibliographic Details
Main Authors:	Larsson, M, Jin, X, Ramratnam, B, Ogg, G, Engelmayer, J, Demoitie, M, Mcmichael, A, Cox, W, Steinman, R, Nixon, D, Bhardwaj, N
Format:	Journal article
Language:	English
Published:	1999

Similar Items

Modified vaccinia Ankara expressing HIVA antigen stimulates HIV-1-specific CD8 T cells in ELISpot assays of HIV-1 exposed infants.
by: Slyker, J, et al.
Published: (2005)

Cultured ELISpot assay to investigate dengue virus specific T-cell responses
by: Jeewandara, C, et al.
Published: (2018)

Increased detection of proliferating, polyfunctional, HIV-1-specific T cells in DNA-modified vaccinia virus Ankara-vaccinated human volunteers by cultured IFN-gamma ELISPOT assay.
by: Winstone, N, et al.
Published: (2009)

HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides.
by: Nixon, D, et al.
Published: (1988)

Immune response in acute hepatitis C: Enumeration of virus specific CD8+T cells with ELISPOT assay and tetramers
by: Gruener, N, et al.
Published: (1999)

ELISPOT Cell Analysis Assay: Searching for Extracellular Footprints
by: Alexander E. Kalyuzhny
Published: (2011-09-01)

Equivalence of ELISpot assays demonstrated between major HIV network laboratories.
by: Dilbinder K Gill, et al.
Published: (2010-12-01)

Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT
by: Magdalena Tary-Lehmann, et al.
Published: (2012-05-01)

Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays
by: Sylvia Janetzki, et al.
Published: (2014-11-01)

Role of ELISPOT Assays in Risk Assessment Pre- and Post-Kidney Transplantation
by: Anat R. Tambur, et al.
Published: (2012-05-01)

Development of an EliSPOT assay for detection of CMV-specific immune response
by: Maria Elena Terlizzi, et al.
Published: (2010-06-01)

ELISPOT assay of the SARS-CoV-2 specific T cells immune response
by: V. V. Gerasimova, et al.
Published: (2022-12-01)

ELISPOT Assay for Monitoring Cytotoxic T Lymphocytes (CTL) Activity in Cancer Vaccine Clinical Trials
by: Thomas J. Sayers, et al.
Published: (2012-05-01)

Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot: Variability of the memory B cell ELISpot assay.
by: Trück, J, et al.
Published: (2014)

Cytotoxic T-cell response to simian immunodeficiency virus by cynomolgus macaque monkeys immunized with recombinant vaccinia virus.
by: Gotch, F, et al.
Published: (1991)

An Enhanced ELISPOT Assay for Sensitive Detection of Antigen-Specific T Cell Responses to Borrelia burgdorferi
by: Gottfried H. Kellermann, et al.
Published: (2013-09-01)

Comparision of Tuberculin Skin Test and RD1-ELISPOT Assay in Health Care Workers
by: Ahmet SOYSAL, et al.
Published: (2005-09-01)

Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay
by: Baseler Michael, et al.
Published: (2004-09-01)

Live-Cell Imaging of Vaccinia Virus Recombination.
by: Patrick Paszkowski, et al.
Published: (2016-08-01)

Recombinant modified vaccinia virus Ankara efficiently restimulates human cytotoxic T lymphocytes in vitro.
by: Dorrell, L, et al.
Published: (2000)

ELISPOTs Produced by CD8 and CD4 Cells Follow Log Normal Size Distribution Permitting Objective Counting
by: Alexey Y. Karulin, et al.
Published: (2015-01-01)

Quantitation of antigen-specific CD8+ T-cell responses.
by: Ogg, G, et al.
Published: (1999)

The Granzyme B ELISPOT assay: an alternative to the <sup>51</sup>Cr-release assay for monitoring cell-mediated cytotoxicity
by: Baseler Michael, et al.
Published: (2003-12-01)

Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay.
by: Grant, C, et al.
Published: (2012)

Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays
by: Ramu A. Subbramanian, et al.
Published: (2012-07-01)

ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells
by: Jodi Hanson, et al.
Published: (2015-01-01)

A modified human ELISPOT assay to detect specific responses to primary tumor cell targets
by: Kwak Larry W, et al.
Published: (2004-03-01)

Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay
by: Grant Clare FJ, et al.
Published: (2012-10-01)

ELISPOT and functional T cell analyses using HLA mono-specific target cells.
by: Horlock, C, et al.
Published: (2009)

Cytomegalovirus (CMV) immune monitoring with ELISPOT and QuantiFERON-CMV assay in seropositive kidney transplant recipients.
by: Hyeyoung Lee, et al.
Published: (2017-01-01)

Sensitivity and specificity of tritiated thymidine incorporation and ELISPOT assays in identifying antigen specific T cell immune responses
by: MacLeod Beth, et al.
Published: (2007-09-01)

Diagnosis of Coxiella burnetii infection: comparison of a whole blood interferon-gamma production assay and a Coxiella ELISPOT.
by: Teske Schoffelen, et al.
Published: (2014-01-01)

Cross-validation of IFN-γ Elispot assay for measuring alloreactive memory/effector T cell responses in renal transplant recipients.
by: Bestard, O, et al.
Published: (2013)

Cytomegalovirus (CMV)-Specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease
by: Paul V. Lehmann, et al.
Published: (2012-04-01)

Novel sporozoite-based ELISpot assay to assess frequency of parasite-specific B cells after vaccination with irradiated sporozoites
by: Tanmaya Atre, et al.
Published: (2019-05-01)

Establishment of an ELISpot Assay to Detect Cellular Immunity against <i>S. pneumoniae</i> in Vaccinated Kidney Transplant Recipients
by: Anja Gäckler, et al.
Published: (2021-12-01)

Distinguishing Latent from Active Mycobacterium tuberculosis Infection Using Elispot Assays: Looking Beyond Interferon-gamma
by: Jennifer E. Snyder-Cappione, et al.
Published: (2012-05-01)

Novel M. tuberculosis specific IL-2 ELISpot assay discriminates adult patients with active or latent tuberculosis.
by: Chiara Della Bella, et al.
Published: (2018-01-01)

Strong human immunodeficiency virus (HIV)-specific cytotoxic T-lymphocyte activity in Sydney Blood Bank Cohort patients infected with nef-defective HIV type 1.
by: Dyer, W, et al.
Published: (1999)

Immunisation with recombinant modified vaccinia virus Ankara expressing HIV-1 gag in HIV-1-infected subjects stimulates broad functional CD4+ T cell responses.
by: Ondondo, B, et al.
Published: (2006)