Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification
Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging,...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Oxford University Press
2021
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| _version_ | 1826308613961940992 |
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| author | Baker, YR Yuan, L Chen, J Belle, R Carlisle, R El-Sagheer, AH Brown, T |
| author_facet | Baker, YR Yuan, L Chen, J Belle, R Carlisle, R El-Sagheer, AH Brown, T |
| author_sort | Baker, YR |
| collection | OXFORD |
| description | Rolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present orthogonal strategies to produce densely modified RCA products and DNFs. We provide methods to selectively modify the DNA component and/or the protein cargo of these materials, thereby greatly expanding the range of chemical functionalities available to these systems. We have used our methodology to construct DNFs bearing multiple surface aptamers and peptides capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating their potential for diagnostic and therapeutic applications. |
| first_indexed | 2024-03-07T07:21:57Z |
| format | Journal article |
| id | oxford-uuid:dd15d56b-5b13-49f6-85dd-b22367616d41 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:21:57Z |
| publishDate | 2021 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:dd15d56b-5b13-49f6-85dd-b22367616d412022-10-11T11:06:03ZExpanding the chemical functionality of DNA nanomaterials generated by rolling circle amplificationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:dd15d56b-5b13-49f6-85dd-b22367616d41EnglishSymplectic ElementsOxford University Press2021Baker, YRYuan, LChen, JBelle, RCarlisle, REl-Sagheer, AHBrown, TRolling circle amplification (RCA) is a powerful tool for the construction of DNA nanomaterials such as hydrogels, high-performance scaffolds and DNA nanoflowers (DNFs), hybrid materials formed of DNA and magnesium pyrophosphate. Such DNA nanomaterials have great potential in therapeutics, imaging, protein immobilisation, and drug delivery, yet limited chemistry is available to expand their functionality. Here, we present orthogonal strategies to produce densely modified RCA products and DNFs. We provide methods to selectively modify the DNA component and/or the protein cargo of these materials, thereby greatly expanding the range of chemical functionalities available to these systems. We have used our methodology to construct DNFs bearing multiple surface aptamers and peptides capable of binding to cancer cells that overexpress the HER2 oncobiomarker, demonstrating their potential for diagnostic and therapeutic applications. |
| spellingShingle | Baker, YR Yuan, L Chen, J Belle, R Carlisle, R El-Sagheer, AH Brown, T Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification |
| title | Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification |
| title_full | Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification |
| title_fullStr | Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification |
| title_full_unstemmed | Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification |
| title_short | Expanding the chemical functionality of DNA nanomaterials generated by rolling circle amplification |
| title_sort | expanding the chemical functionality of dna nanomaterials generated by rolling circle amplification |
| work_keys_str_mv | AT bakeryr expandingthechemicalfunctionalityofdnananomaterialsgeneratedbyrollingcircleamplification AT yuanl expandingthechemicalfunctionalityofdnananomaterialsgeneratedbyrollingcircleamplification AT chenj expandingthechemicalfunctionalityofdnananomaterialsgeneratedbyrollingcircleamplification AT beller expandingthechemicalfunctionalityofdnananomaterialsgeneratedbyrollingcircleamplification AT carlisler expandingthechemicalfunctionalityofdnananomaterialsgeneratedbyrollingcircleamplification AT elsagheerah expandingthechemicalfunctionalityofdnananomaterialsgeneratedbyrollingcircleamplification AT brownt expandingthechemicalfunctionalityofdnananomaterialsgeneratedbyrollingcircleamplification |