The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase.
The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for...
| Egile Nagusiak: | , , , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
2004
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| _version_ | 1826300348245999616 |
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| author | Salgado, P Makeyev, E Butcher, S Bamford, D Stuart, D Grimes, J |
| author_facet | Salgado, P Makeyev, E Butcher, S Bamford, D Stuart, D Grimes, J |
| author_sort | Salgado, P |
| collection | OXFORD |
| description | The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+. |
| first_indexed | 2024-03-07T05:15:46Z |
| format | Journal article |
| id | oxford-uuid:dd1ff4b2-ef6b-486a-b434-09450be86ec3 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:15:46Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:dd1ff4b2-ef6b-486a-b434-09450be86ec32022-03-27T09:22:50ZThe structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:dd1ff4b2-ef6b-486a-b434-09450be86ec3EnglishSymplectic Elements at Oxford2004Salgado, PMakeyev, EButcher, SBamford, DStuart, DGrimes, JThe RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+. |
| spellingShingle | Salgado, P Makeyev, E Butcher, S Bamford, D Stuart, D Grimes, J The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. |
| title | The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. |
| title_full | The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. |
| title_fullStr | The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. |
| title_full_unstemmed | The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. |
| title_short | The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. |
| title_sort | structural basis for rna specificity and ca2 inhibition of an rna dependent rna polymerase |
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