Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation.
Cep135/Bld10 is a conserved centriolar protein required for the formation of the central cartwheel, an early intermediate in centriole assembly. Surprisingly, Cep135/Bld10 is not essential for centriole duplication in Drosophila, suggesting either that Cep135/Bld10 is not essential for cartwheel for...
| Egile Nagusiak: | , , , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
2012
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| _version_ | 1826300351922307072 |
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| author | Roque, H Wainman, A Richens, J Kozyrska, K Franz, A Raff, J |
| author_facet | Roque, H Wainman, A Richens, J Kozyrska, K Franz, A Raff, J |
| author_sort | Roque, H |
| collection | OXFORD |
| description | Cep135/Bld10 is a conserved centriolar protein required for the formation of the central cartwheel, an early intermediate in centriole assembly. Surprisingly, Cep135/Bld10 is not essential for centriole duplication in Drosophila, suggesting either that Cep135/Bld10 is not essential for cartwheel formation, or that the cartwheel is not essential for centriole assembly in flies. Using electron tomography and super-resolution microscopy we show that centrioles can form a cartwheel in the absence of Cep135/Bld10, but centriole width is increased and the cartwheel appears to disassemble over time. Using 3D structured illumination microscopy we show that Cep135/Bld10 is localized to a region between inner (SAS-6, Ana2) and outer (Asl, DSpd-2 and D-PLP) centriolar components, and the localization of all these component is subtly perturbed in the absence of Cep135/Bld10, although the ninefold symmetry of the centriole is maintained. Thus, in flies, Cep135/Bld10 is not essential for cartwheel assembly or for establishing the ninefold symmetry of centrioles; rather, it appears to stabilize the connection between inner and outer centriole components. |
| first_indexed | 2024-03-07T05:15:50Z |
| format | Journal article |
| id | oxford-uuid:dd24b4a2-4af9-4e6b-ae79-132d76a15a0e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:15:50Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:dd24b4a2-4af9-4e6b-ae79-132d76a15a0e2022-03-27T09:23:03ZDrosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:dd24b4a2-4af9-4e6b-ae79-132d76a15a0eEnglishSymplectic Elements at Oxford2012Roque, HWainman, ARichens, JKozyrska, KFranz, ARaff, JCep135/Bld10 is a conserved centriolar protein required for the formation of the central cartwheel, an early intermediate in centriole assembly. Surprisingly, Cep135/Bld10 is not essential for centriole duplication in Drosophila, suggesting either that Cep135/Bld10 is not essential for cartwheel formation, or that the cartwheel is not essential for centriole assembly in flies. Using electron tomography and super-resolution microscopy we show that centrioles can form a cartwheel in the absence of Cep135/Bld10, but centriole width is increased and the cartwheel appears to disassemble over time. Using 3D structured illumination microscopy we show that Cep135/Bld10 is localized to a region between inner (SAS-6, Ana2) and outer (Asl, DSpd-2 and D-PLP) centriolar components, and the localization of all these component is subtly perturbed in the absence of Cep135/Bld10, although the ninefold symmetry of the centriole is maintained. Thus, in flies, Cep135/Bld10 is not essential for cartwheel assembly or for establishing the ninefold symmetry of centrioles; rather, it appears to stabilize the connection between inner and outer centriole components. |
| spellingShingle | Roque, H Wainman, A Richens, J Kozyrska, K Franz, A Raff, J Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. |
| title | Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. |
| title_full | Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. |
| title_fullStr | Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. |
| title_full_unstemmed | Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. |
| title_short | Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation. |
| title_sort | drosophila cep135 bld10 maintains proper centriole structure but is dispensable for cartwheel formation |
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