Dot-blot hybridisation assay for detection of parvovirus B19 infections using synthetic oligonucleotides.
Pools of 10 synthetic oligonucleotides with sequences derived from the genome of parvovirus B19 and of 30 bases in length were made and labelled at the time of synthesis with digoxigenin (DIG) or dinitrophenyl (DNP) at the 5' end. They were used in a dot-blot hybridisation assay to detect parvo...
Main Authors: | Cubie, H, Molyneaux, P, Shearman, M, Gryzbowski, J, Brown, T |
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Format: | Journal article |
Language: | English |
Published: |
1995
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