Measurement of T cell alloreactivity using imaging flow cytometry

Measurement of T cell alloreactivity using imaging flow cytometry

The measurement of immunological reactivity to donor antigens in transplant recipients is likely to be crucial for the successful reduction or withdrawal of immunosuppression. The mixed leukocyte reaction (MLR), limiting dilution assays, and trans-vivo delayed-type hypersensitivity (DTH) assay have...

Full description

Bibliographic Details
Main Authors:	Juvet, S, Moshkelgosha, S, Sanderson, S, Hester, J, Wood, K, Bushell, A
Format:	Journal article
Language:	English
Published:	Journal of Visualised Experiments 2017

Similar Items

Quantification of CD4(+) T cell alloreactivity and its control by regulatory T cells using time lapse microscopy and immune synapse detection.
by: Juvet, S, et al.
Published: (2015)

Alloreactive regulatory T cells generated with retinoic acid prevent skin allograft rejection.
by: Moore, C, et al.
Published: (2015)

Metabolic Pathways in Alloreactive T Cells
by: Rebecca A. Brown, et al.
Published: (2020-07-01)

Exogenous IFN-gamma ex vivo shapes the alloreactive T-cell repertoire by inhibition of Th17 responses and generation of functional Foxp3+ regulatory T cells.
by: Feng, G, et al.
Published: (2008)

In aged primary T cells mitochondrial stress contributes to telomere attrition measured by a novel imaging flow cytometry assay
by: Simon, AK, et al.
Published: (2017)

Harnessing regulatory t cells for clinical use in transplantation: The end of the beginning
by: Juvet, S, et al.
Published: (2014)

Harnessing regulatory T cells for clinical use in transplantation: the end of the beginning.
by: Juvet, S, et al.
Published: (2014)

Imaging alloreactive T cells provides early warning of organ transplant rejection
by: Toshihito Hirai, et al.
Published: (2021-07-01)

Comparison of an image cytometry somatic cell count analyzer to a flow cytometry analyzer
by: A. Hubner, et al.
Published: (2023-11-01)

Flow cytometry and integrated imaging
by: V. Kachel, et al.
Published: (2000-06-01)

Exhaustive differentiation of alloreactive CD8+ T cells: critical for determination of graft acceptance or rejection.
by: Steger, U, et al.
Published: (2008)

Embryonic stem cells: protecting pluripotency from alloreactivity.
by: Fairchild, P, et al.
Published: (2007)

Ex Vivo Assessment of Transplantation Tolerance Using Time Lapse Microscopy
by: Juvet, S, et al.
Published: (2014)

Ex Vivo Assessment of Transplantation Tolerance Using Time Lapse Microscopy
by: Juvet, S, et al.
Published: (2014)

Cross-validation of IFN-γ Elispot assay for measuring alloreactive memory/effector T cell responses in renal transplant recipients.
by: Bestard, O, et al.
Published: (2013)

Regulatory immune cells in transplantation.
by: Wood, K, et al.
Published: (2012)

Quantitative measurement of mammalian cell invasion by bacteria using flow cytometry
by: Lower, Michal, et al.
Published: (2010)

Detection of cells by flow cytometry: Counting, imaging, and cell classification
by: Yingsi Yu, et al.
Published: (2023-05-01)

Flow cytometry and cell sorting /
by: Radbruch, A. (Andreas)
Published: (2000)

Flow cytometry /
by: Darzynkiewicz, Zbigniew, et al.
Published: (1994)

Artificial intelligence in imaging flow cytometry
by: Paolo Pozzi, et al.
Published: (2023-10-01)

Using Flow Cytometry to Measure Phagocytic Uptake in Earthworms
by: Sheryl L. Fuller-Espie
Published: (2010-01-01)

Alloreactive T cells to Assess Acute Rejection Risk in Kidney Transplant Recipients
by: Aleixandra Mendoza Rojas, MSc, et al.
Published: (2023-05-01)

Mimotopes for alloreactive and conventional T cells in a peptide-MHC display library.
by: Frances Crawford, et al.
Published: (2004-04-01)

Alloreactive regulatory T cells allow the generation of mixed chimerism and transplant tolerance
by: Paulina eRuiz, et al.
Published: (2015-11-01)

Flow cytometry and cell sorting
by: William G. Telford
Published: (2023-11-01)

Cell Tracking By Gadolinium Labelling and Mass Spectrometry:-A Tool for Evaluating The Persistence of Cellular Therapies in Transplantation
by: Bushell, A, et al.
Published: (2014)

Cell Tracking By Gadolinium Labelling and Mass Spectrometry:- A Tool for Evaluating The Persistence of Cellular Therapies in Transplantation.
by: Bushell, A, et al.
Published: (2014)

Delayed anti-CD3 therapy results in depletion of alloreactive T cells and the dominance of Foxp3+ CD4+ graft infiltrating cells.
by: Goto, R, et al.
Published: (2013)

Delayed Anti-CD3 Therapy Results in Depletion of Alloreactive T Cells and the Dominance of Foxp3+CD4+Graft Infiltrating Cells
by: Goto, R, et al.
Published: (2013)

Immunological aspects of unrelated bone marrow transplantation: alloreactivity and immunoreconstitution
by: Madrigal J. Alejandro
Published: (2001-01-01)

Flow cytometry in the study of cell death
by: Álvaro L Bertho, et al.
Published: (2000-06-01)

Virtual-freezing fluorescence imaging flow cytometry
by: Hideharu Mikami, et al.
Published: (2020-03-01)

Guidelines for the use of flow cytometry and cell sorting in immunological studies.
by: Cossarizza, A, et al.
Published: (2017)

Calibration-Aimed Comparison of Image-Cytometry- and Flow-Cytometry-Based Approaches of Ploidy Analysis
by: Viktor Zoltán Jónás, et al.
Published: (2022-09-01)

Defining the alloreactive T cell repertoire using high-throughput sequencing of mixed lymphocyte reaction culture.
by: Ryan O Emerson, et al.
Published: (2014-01-01)

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry
by: Richard Gerum, et al.
Published: (2022-09-01)

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements.
by: William Moutier, et al.
Published: (2017-01-01)

Mammalian cell clonal growth and secretion measurements using gel microdroplets and flow cytometry
by: Powell, Kevin
Published: (2005)

Simple Detection of Unstained Live Senescent Cells with Imaging Flow Cytometry
by: Marco Malavolta, et al.
Published: (2022-08-01)