Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses.
In recent years, the development of new technologies based on the green fluorescent protein and fluorescence resonance energy transfer has introduced a new perspective in the study of cell biology. Real-time imaging of fluorescent biosensors has made it possible to directly visualize individual mole...
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1797099083642437632 |
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| author | Zaccolo, M |
| author_facet | Zaccolo, M |
| author_sort | Zaccolo, M |
| collection | OXFORD |
| description | In recent years, the development of new technologies based on the green fluorescent protein and fluorescence resonance energy transfer has introduced a new perspective in the study of cell biology. Real-time imaging of fluorescent biosensors has made it possible to directly visualize individual molecular events as they happen in intact, live cells, providing important and original insights for our understanding of biologically relevant problems. This review discusses some essential methodological aspects concerning the generation and use of fluorescence resonance energy transfer-based biosensors and presents selected examples of specific applications that highlight the power of this technology. |
| first_indexed | 2024-03-07T05:18:35Z |
| format | Journal article |
| id | oxford-uuid:de194977-7930-40de-b40f-47b8a36c60d4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:18:35Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:de194977-7930-40de-b40f-47b8a36c60d42022-03-27T09:29:40ZUse of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:de194977-7930-40de-b40f-47b8a36c60d4EnglishSymplectic Elements at Oxford2004Zaccolo, MIn recent years, the development of new technologies based on the green fluorescent protein and fluorescence resonance energy transfer has introduced a new perspective in the study of cell biology. Real-time imaging of fluorescent biosensors has made it possible to directly visualize individual molecular events as they happen in intact, live cells, providing important and original insights for our understanding of biologically relevant problems. This review discusses some essential methodological aspects concerning the generation and use of fluorescence resonance energy transfer-based biosensors and presents selected examples of specific applications that highlight the power of this technology. |
| spellingShingle | Zaccolo, M Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses. |
| title | Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses. |
| title_full | Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses. |
| title_fullStr | Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses. |
| title_full_unstemmed | Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses. |
| title_short | Use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses. |
| title_sort | use of chimeric fluorescent proteins and fluorescence resonance energy transfer to monitor cellular responses |
| work_keys_str_mv | AT zaccolom useofchimericfluorescentproteinsandfluorescenceresonanceenergytransfertomonitorcellularresponses |