Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression.
We provide evidence for a novel mechanism of transcriptional regulation in which the immediate-early (IE) transactivating protein of herpes simplex virus, Vmw65, is assembled into a specific DNA-binding complex together with a cellular octamer-binding factor (TRF). The assembly of Vmw65/TRF complex...
| Prif Awduron: | , , |
|---|---|
| Fformat: | Journal article |
| Iaith: | English |
| Cyhoeddwyd: |
1988
|
| _version_ | 1826300639935725568 |
|---|---|
| author | O'Hare, P Goding, C Haigh, A |
| author_facet | O'Hare, P Goding, C Haigh, A |
| author_sort | O'Hare, P |
| collection | OXFORD |
| description | We provide evidence for a novel mechanism of transcriptional regulation in which the immediate-early (IE) transactivating protein of herpes simplex virus, Vmw65, is assembled into a specific DNA-binding complex together with a cellular octamer-binding factor (TRF). The assembly of Vmw65/TRF complex requires not only the core TRF recognition site, but also flanking sequences which are dispensable for TRF binding alone. We show from functional analyses that TRF binding by a motif is required but not sufficient to confer induction on a heterologous promoter, and it is the ability of the motif to allow TRF/Vmw65 complex assembly which correlates with functional activity. Thus, for the induction of HSV IE expression, Vmw65 forms a complex with TRF by recognition of the specific subset of appropriately flanked TRF binding sites present in each of the IE genes. This mechanism may provide a paradigm for the selective utilization of the same transcription factor in differential gene expression. |
| first_indexed | 2024-03-07T05:20:15Z |
| format | Journal article |
| id | oxford-uuid:dea1e89f-e351-4bbd-ad6f-565842aed6c0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:20:15Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:dea1e89f-e351-4bbd-ad6f-565842aed6c02022-03-27T09:33:40ZDirect combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:dea1e89f-e351-4bbd-ad6f-565842aed6c0EnglishSymplectic Elements at Oxford1988O'Hare, PGoding, CHaigh, AWe provide evidence for a novel mechanism of transcriptional regulation in which the immediate-early (IE) transactivating protein of herpes simplex virus, Vmw65, is assembled into a specific DNA-binding complex together with a cellular octamer-binding factor (TRF). The assembly of Vmw65/TRF complex requires not only the core TRF recognition site, but also flanking sequences which are dispensable for TRF binding alone. We show from functional analyses that TRF binding by a motif is required but not sufficient to confer induction on a heterologous promoter, and it is the ability of the motif to allow TRF/Vmw65 complex assembly which correlates with functional activity. Thus, for the induction of HSV IE expression, Vmw65 forms a complex with TRF by recognition of the specific subset of appropriately flanked TRF binding sites present in each of the IE genes. This mechanism may provide a paradigm for the selective utilization of the same transcription factor in differential gene expression. |
| spellingShingle | O'Hare, P Goding, C Haigh, A Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. |
| title | Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. |
| title_full | Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. |
| title_fullStr | Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. |
| title_full_unstemmed | Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. |
| title_short | Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. |
| title_sort | direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer binding factor mediates specific induction of virus immediate early gene expression |
| work_keys_str_mv | AT oharep directcombinatorialinteractionbetweenaherpessimplexvirusregulatoryproteinandacellularoctamerbindingfactormediatesspecificinductionofvirusimmediateearlygeneexpression AT godingc directcombinatorialinteractionbetweenaherpessimplexvirusregulatoryproteinandacellularoctamerbindingfactormediatesspecificinductionofvirusimmediateearlygeneexpression AT haigha directcombinatorialinteractionbetweenaherpessimplexvirusregulatoryproteinandacellularoctamerbindingfactormediatesspecificinductionofvirusimmediateearlygeneexpression |