Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis.
OBJECTIVE: To examine peripheral leukocyte Dectin-1 regulation in clinically relevant models of fungal and polymicrobial sepsis. DESIGN: Prospective animal study. SETTING: University medical school research laboratory. SUBJECTS: Age, weight, and sex matched ICR/HSD mice. INTERVENTIONS: Mice were inf...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2009
|
| _version_ | 1826300680290172928 |
|---|---|
| author | Ozment-Skelton, T deFluiter, E Ha, T Li, C Graves, B Ferguson, D Schweitzer, J Preizsner, J Brown, G Gordon, S Kalbfleisch, J Williams, D |
| author_facet | Ozment-Skelton, T deFluiter, E Ha, T Li, C Graves, B Ferguson, D Schweitzer, J Preizsner, J Brown, G Gordon, S Kalbfleisch, J Williams, D |
| author_sort | Ozment-Skelton, T |
| collection | OXFORD |
| description | OBJECTIVE: To examine peripheral leukocyte Dectin-1 regulation in clinically relevant models of fungal and polymicrobial sepsis. DESIGN: Prospective animal study. SETTING: University medical school research laboratory. SUBJECTS: Age, weight, and sex matched ICR/HSD mice. INTERVENTIONS: Mice were infected with Candida albicans (1 x 10, intravenously) or were subjected to cecal ligation and puncture to induce polymicrobial sepsis. MEASUREMENTS: Blood, spleen, and peritoneal exudate were harvested and leukocytes were isolated. Leukocytes were evaluated for membrane-associated Dectin-1 expression and cell phenotype by flow cytometry. MAIN RESULTS: In C. albicans infection, Dectin-1-positive blood and splenic leukocytes were increased from 23.5% to 58.9% over the course of infection. The increased percentage of Dectin-1-expressing cells was primarily attributable to neutrophilia. However, the amount of Dectin-1 expressed by blood and splenic neutrophils in C. albicans-infected mice was decreased by a range of 49.0% to 53.3%. C. albicans infection also resulted in an infiltration of Dectin-1-positive macrophages and neutrophils into the kidney. In contrast, polymicrobial sepsis decreased blood leukocyte Dectin-1-expressing cells by up to 51.4%. This reduction was due to a decrease in Dectin-1-positive neutrophils in the periphery. However, the percentage of Dectin-1-expressing cells in the peritoneal cavity increased by 774% with cecal ligation and puncture. Treatment of isolated neutrophils with three soluble glucans, mannan, lipopolysaccharide, or a variety of cytokines revealed that glucans, alone or in combination, were the only treatment that resulted in a decrease in Dectin-1-positive neutrophils. CONCLUSIONS: We conclude that peripheral leukocyte Dectin-1 expression is differentially regulated in fungal vs. polymicrobial sepsis. These data demonstrate that leukocyte Dectin-1 levels are modulated in response to infections of fungal and nonfungal origin. |
| first_indexed | 2024-03-07T05:20:50Z |
| format | Journal article |
| id | oxford-uuid:ded739f0-7433-4a4f-b4f5-2e95e62e9dc9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:20:50Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:ded739f0-7433-4a4f-b4f5-2e95e62e9dc92022-03-27T09:35:07ZLeukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ded739f0-7433-4a4f-b4f5-2e95e62e9dc9EnglishSymplectic Elements at Oxford2009Ozment-Skelton, TdeFluiter, EHa, TLi, CGraves, BFerguson, DSchweitzer, JPreizsner, JBrown, GGordon, SKalbfleisch, JWilliams, DOBJECTIVE: To examine peripheral leukocyte Dectin-1 regulation in clinically relevant models of fungal and polymicrobial sepsis. DESIGN: Prospective animal study. SETTING: University medical school research laboratory. SUBJECTS: Age, weight, and sex matched ICR/HSD mice. INTERVENTIONS: Mice were infected with Candida albicans (1 x 10, intravenously) or were subjected to cecal ligation and puncture to induce polymicrobial sepsis. MEASUREMENTS: Blood, spleen, and peritoneal exudate were harvested and leukocytes were isolated. Leukocytes were evaluated for membrane-associated Dectin-1 expression and cell phenotype by flow cytometry. MAIN RESULTS: In C. albicans infection, Dectin-1-positive blood and splenic leukocytes were increased from 23.5% to 58.9% over the course of infection. The increased percentage of Dectin-1-expressing cells was primarily attributable to neutrophilia. However, the amount of Dectin-1 expressed by blood and splenic neutrophils in C. albicans-infected mice was decreased by a range of 49.0% to 53.3%. C. albicans infection also resulted in an infiltration of Dectin-1-positive macrophages and neutrophils into the kidney. In contrast, polymicrobial sepsis decreased blood leukocyte Dectin-1-expressing cells by up to 51.4%. This reduction was due to a decrease in Dectin-1-positive neutrophils in the periphery. However, the percentage of Dectin-1-expressing cells in the peritoneal cavity increased by 774% with cecal ligation and puncture. Treatment of isolated neutrophils with three soluble glucans, mannan, lipopolysaccharide, or a variety of cytokines revealed that glucans, alone or in combination, were the only treatment that resulted in a decrease in Dectin-1-positive neutrophils. CONCLUSIONS: We conclude that peripheral leukocyte Dectin-1 expression is differentially regulated in fungal vs. polymicrobial sepsis. These data demonstrate that leukocyte Dectin-1 levels are modulated in response to infections of fungal and nonfungal origin. |
| spellingShingle | Ozment-Skelton, T deFluiter, E Ha, T Li, C Graves, B Ferguson, D Schweitzer, J Preizsner, J Brown, G Gordon, S Kalbfleisch, J Williams, D Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. |
| title | Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. |
| title_full | Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. |
| title_fullStr | Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. |
| title_full_unstemmed | Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. |
| title_short | Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. |
| title_sort | leukocyte dectin 1 expression is differentially regulated in fungal versus polymicrobial sepsis |
| work_keys_str_mv | AT ozmentskeltont leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT defluitere leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT hat leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT lic leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT gravesb leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT fergusond leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT schweitzerj leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT preizsnerj leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT browng leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT gordons leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT kalbfleischj leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis AT williamsd leukocytedectin1expressionisdifferentiallyregulatedinfungalversuspolymicrobialsepsis |