Label-free methods for optical in vitro characterization of protein–protein interactions
Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Royal Society of Chemistry
2021
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| _version_ | 1797099247337734144 |
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| author | Soltermann, F Struwe, WB Kukura, P |
| author_facet | Soltermann, F Struwe, WB Kukura, P |
| author_sort | Soltermann, F |
| collection | OXFORD |
| description | Protein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications. |
| first_indexed | 2024-03-07T05:21:02Z |
| format | Journal article |
| id | oxford-uuid:dee99e47-6bdf-42b3-90f4-c5cfd08e772e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:21:02Z |
| publishDate | 2021 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:dee99e47-6bdf-42b3-90f4-c5cfd08e772e2022-03-27T09:35:35ZLabel-free methods for optical in vitro characterization of protein–protein interactionsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:dee99e47-6bdf-42b3-90f4-c5cfd08e772eEnglishSymplectic ElementsRoyal Society of Chemistry2021Soltermann, FStruwe, WBKukura, PProtein–protein interactions are involved in the regulation and function of the majority of cellular processes. As a result, much effort has been aimed at the development of methodologies capable of quantifying protein–protein interactions, with label-free methods being of particular interest due to the associated simplified workflows and minimisation of label-induced perturbations. Here, we review recent advances in optical technologies providing label-free in vitro measurements of affinities and kinetics. We provide an overview and comparison of existing techniques and their principles, discussing advantages, limitations, and recent applications. |
| spellingShingle | Soltermann, F Struwe, WB Kukura, P Label-free methods for optical in vitro characterization of protein–protein interactions |
| title | Label-free methods for optical in vitro characterization of protein–protein interactions |
| title_full | Label-free methods for optical in vitro characterization of protein–protein interactions |
| title_fullStr | Label-free methods for optical in vitro characterization of protein–protein interactions |
| title_full_unstemmed | Label-free methods for optical in vitro characterization of protein–protein interactions |
| title_short | Label-free methods for optical in vitro characterization of protein–protein interactions |
| title_sort | label free methods for optical in vitro characterization of protein protein interactions |
| work_keys_str_mv | AT soltermannf labelfreemethodsforopticalinvitrocharacterizationofproteinproteininteractions AT struwewb labelfreemethodsforopticalinvitrocharacterizationofproteinproteininteractions AT kukurap labelfreemethodsforopticalinvitrocharacterizationofproteinproteininteractions |