The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication.
Nuclear DNA is looped by attachment to a matrix or cage. As this cage is the site of DNA synthesis, sequences in the loops must attach before they are replicated. We have tested whether sequences which initiate replication are usually out in the loop and attach only during S phase or whether they ar...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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1984
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| _version_ | 1826300772967514112 |
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| author | Cook, P Lang, J |
| author_facet | Cook, P Lang, J |
| author_sort | Cook, P |
| collection | OXFORD |
| description | Nuclear DNA is looped by attachment to a matrix or cage. As this cage is the site of DNA synthesis, sequences in the loops must attach before they are replicated. We have tested whether sequences which initiate replication are usually out in the loop and attach only during S phase or whether they are attached but quiescent during most of the cell-cycle. Sequences which permit plasmids to replicate autonomously in yeast cells (ARS's) are strong candidates for initiating sequences. Four different human ARS's all map remote from attachment points to the HeLa nuclear cage. In addition a potential terminus of replication is also remote from the cage. We conclude that sequences involved in initiation are usually out in the loop and that DNA synthesis is initiated by their attachment. |
| first_indexed | 2024-03-07T05:22:15Z |
| format | Journal article |
| id | oxford-uuid:df53bd14-b139-4ef9-b1a0-da8a49aa35e6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:22:15Z |
| publishDate | 1984 |
| record_format | dspace |
| spelling | oxford-uuid:df53bd14-b139-4ef9-b1a0-da8a49aa35e62022-03-27T09:38:33ZThe spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:df53bd14-b139-4ef9-b1a0-da8a49aa35e6EnglishSymplectic Elements at Oxford1984Cook, PLang, JNuclear DNA is looped by attachment to a matrix or cage. As this cage is the site of DNA synthesis, sequences in the loops must attach before they are replicated. We have tested whether sequences which initiate replication are usually out in the loop and attach only during S phase or whether they are attached but quiescent during most of the cell-cycle. Sequences which permit plasmids to replicate autonomously in yeast cells (ARS's) are strong candidates for initiating sequences. Four different human ARS's all map remote from attachment points to the HeLa nuclear cage. In addition a potential terminus of replication is also remote from the cage. We conclude that sequences involved in initiation are usually out in the loop and that DNA synthesis is initiated by their attachment. |
| spellingShingle | Cook, P Lang, J The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication. |
| title | The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication. |
| title_full | The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication. |
| title_fullStr | The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication. |
| title_full_unstemmed | The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication. |
| title_short | The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication. |
| title_sort | spatial organization of sequences involved in initiation and termination of eukaryotic dna replication |
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