Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines.
The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. Beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CAT...
| Egile Nagusiak: | , , , , , |
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| Formatua: | Journal article |
| Hizkuntza: | English |
| Argitaratua: |
2004
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| _version_ | 1826300778822762496 |
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| author | El-Bahrawy, M Poulsom, R Rowan, A Tomlinson, I Alison, MR Poulsom, SR Tomlinson, I |
| author_facet | El-Bahrawy, M Poulsom, R Rowan, A Tomlinson, I Alison, MR Poulsom, SR Tomlinson, I |
| author_sort | El-Bahrawy, M |
| collection | OXFORD |
| description | The E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. Beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations. |
| first_indexed | 2024-03-07T05:22:20Z |
| format | Journal article |
| id | oxford-uuid:df58fdde-7fb0-4d25-85a2-89968a80c52f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:22:20Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:df58fdde-7fb0-4d25-85a2-89968a80c52f2022-03-27T09:38:51ZCharacterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:df58fdde-7fb0-4d25-85a2-89968a80c52fEnglishSymplectic Elements at Oxford2004El-Bahrawy, MPoulsom, RRowan, ATomlinson, IAlison, MRPoulsom, SRTomlinson, IThe E-cadherin/catenin complex is a prime mediator of cell-cell adhesion. APC mutations can result in loss of beta-catenin downregulation and an accumulation of beta-catenin in the cell. Beta-CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of beta-CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and beta-CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/beta-catenin/alpha-catenin. The subcellular distribution of the proteins is influenced by cell-cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or beta-CATENIN mutations. |
| spellingShingle | El-Bahrawy, M Poulsom, R Rowan, A Tomlinson, I Alison, MR Poulsom, SR Tomlinson, I Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines. |
| title | Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines. |
| title_full | Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines. |
| title_fullStr | Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines. |
| title_full_unstemmed | Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines. |
| title_short | Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines. |
| title_sort | characterization of the e cadherin catenin complex in colorectal carcinoma cell lines |
| work_keys_str_mv | AT elbahrawym characterizationoftheecadherincatenincomplexincolorectalcarcinomacelllines AT poulsomr characterizationoftheecadherincatenincomplexincolorectalcarcinomacelllines AT rowana characterizationoftheecadherincatenincomplexincolorectalcarcinomacelllines AT tomlinsoni characterizationoftheecadherincatenincomplexincolorectalcarcinomacelllines AT alisonmr characterizationoftheecadherincatenincomplexincolorectalcarcinomacelllines AT poulsomsr characterizationoftheecadherincatenincomplexincolorectalcarcinomacelllines AT tomlinsoni characterizationoftheecadherincatenincomplexincolorectalcarcinomacelllines |