Activity-Based Protein Profiling (ABPP) for cellular deubiquitinase (DUB) and inhibitor profiling at deep and high-throughput levels
This chapter provides detailed methodology and materials required to profile deubiquitinases (DUBs) in a cellular matrix using specific activity-based probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of activity-based protein profiling (ABPP) for studying...
| Main Authors: | , , , |
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| Format: | Book section |
| Language: | English |
| Published: |
Springer
2022
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| Summary: | This chapter provides detailed methodology and materials required to profile deubiquitinases (DUBs) in a cellular matrix using specific activity-based probes, with immunoblotting and mass spectrometry proteomics-based readouts. Different types of activity-based protein profiling (ABPP) for studying the potency and selectivity of DUB inhibitors are outlined here, including the standard ABPP, the deep DUBome ABPP, and the ABPP-HT (high-throughput compatible). |
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