Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells.
CD4(+) T cells are thought to be critical in the maintenance of virus-specific CD8(+) cytotoxic T-cell (CTL) responses. In human immunodeficiency virus type 1 (HIV-1) infection, a selective decline in HIV-1-specific CTL as the CD4(+) T-cell count decreases has been reported. Using HLA-peptide tetram...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2000
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| _version_ | 1797099615560925184 |
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| author | Spiegel, H Ogg, G DeFalcon, E Sheehy, M Monard, S Haslett, P Gillespie, G Donahoe, S Pollack, H Borkowsky, W Mcmichael, A Nixon, D |
| author_facet | Spiegel, H Ogg, G DeFalcon, E Sheehy, M Monard, S Haslett, P Gillespie, G Donahoe, S Pollack, H Borkowsky, W Mcmichael, A Nixon, D |
| author_sort | Spiegel, H |
| collection | OXFORD |
| description | CD4(+) T cells are thought to be critical in the maintenance of virus-specific CD8(+) cytotoxic T-cell (CTL) responses. In human immunodeficiency virus type 1 (HIV-1) infection, a selective decline in HIV-1-specific CTL as the CD4(+) T-cell count decreases has been reported. Using HLA-peptide tetrameric complexes, we show the presence at high frequency of HIV-1- and cytomegalovirus-specific CD8(+) T cells when the peripheral CD4(+) T-cell count was low or zero in three HIV-1-infected patients. No direct virus-specific CD8(+)-mediated effector activity was seen in these subjects, suggesting antigen unresponsiveness, although tetramer-sorted cells could be expanded in vitro in the presence of interleukin-2 into responsive effector cells. Thus, virus-specific CD8(+) T cells can be maintained in the peripheral circulation at high frequency in the absence of circulating peripheral CD4(+) T cells, but these cells may lack direct effector activity. Strategies designed to overcome this antigen unresponsiveness may be of value in therapies for the treatment of AIDS. |
| first_indexed | 2024-03-07T05:26:12Z |
| format | Journal article |
| id | oxford-uuid:e0a4a7c1-f555-4b19-a530-206e3fb748fe |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:26:12Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:e0a4a7c1-f555-4b19-a530-206e3fb748fe2022-03-27T09:48:45ZHuman immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e0a4a7c1-f555-4b19-a530-206e3fb748feEnglishSymplectic Elements at Oxford2000Spiegel, HOgg, GDeFalcon, ESheehy, MMonard, SHaslett, PGillespie, GDonahoe, SPollack, HBorkowsky, WMcmichael, ANixon, DCD4(+) T cells are thought to be critical in the maintenance of virus-specific CD8(+) cytotoxic T-cell (CTL) responses. In human immunodeficiency virus type 1 (HIV-1) infection, a selective decline in HIV-1-specific CTL as the CD4(+) T-cell count decreases has been reported. Using HLA-peptide tetrameric complexes, we show the presence at high frequency of HIV-1- and cytomegalovirus-specific CD8(+) T cells when the peripheral CD4(+) T-cell count was low or zero in three HIV-1-infected patients. No direct virus-specific CD8(+)-mediated effector activity was seen in these subjects, suggesting antigen unresponsiveness, although tetramer-sorted cells could be expanded in vitro in the presence of interleukin-2 into responsive effector cells. Thus, virus-specific CD8(+) T cells can be maintained in the peripheral circulation at high frequency in the absence of circulating peripheral CD4(+) T cells, but these cells may lack direct effector activity. Strategies designed to overcome this antigen unresponsiveness may be of value in therapies for the treatment of AIDS. |
| spellingShingle | Spiegel, H Ogg, G DeFalcon, E Sheehy, M Monard, S Haslett, P Gillespie, G Donahoe, S Pollack, H Borkowsky, W Mcmichael, A Nixon, D Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells. |
| title | Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells. |
| title_full | Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells. |
| title_fullStr | Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells. |
| title_full_unstemmed | Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells. |
| title_short | Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells. |
| title_sort | human immunodeficiency virus type 1 and cytomegalovirus specific cytotoxic t lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral cd4 t cells |
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