Two-color far-field fluorescence nanoscopy.

We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The s...

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Detalhes bibliográficos
Principais autores: Donnert, G, Keller, J, Wurm, C, Rizzoli, S, Westphal, V, Schönle, A, Jahn, R, Jakobs, S, Eggeling, C, Hell, S
Formato: Journal article
Idioma:English
Publicado em: 2007