In vitro visualization of cell-to-cell interactions between natural killer cells and sensory neurons
Cell-to-cell interactions between the immune and nervous systems are increasingly recognized for their importance in health and disease. Assessment of cellular neuro-immune interactions can be aided by co-culture of two (or more) cells in an in vitro model system that preserves the morphology of neu...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Book section |
| Language: | English |
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Humana Press
2022
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| _version_ | 1826310658476474368 |
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| author | Kim, HW Davies, A Oh, SB |
| author2 | Shimasaki, N |
| author_facet | Shimasaki, N Kim, HW Davies, A Oh, SB |
| author_sort | Kim, HW |
| collection | OXFORD |
| description | Cell-to-cell interactions between the immune and nervous systems are increasingly recognized for their importance in health and disease. Assessment of cellular neuro-immune interactions can be aided by co-culture of two (or more) cells in an in vitro model system that preserves the morphology of neuronal cells. Here we describe methods to investigate the cytotoxic effector functions of natural killer cells on sensory neurons isolated from syngeneic embryonic and adult mice. We present methods for the morphological analysis of axon fragmentation (pruning) and dynamic cell function via live confocal calcium imaging. These techniques can easily be adapted to study interactions between other combinations of immune cell subsets and neuronal populations. |
| first_indexed | 2024-03-07T07:55:11Z |
| format | Book section |
| id | oxford-uuid:e141bc8d-73ea-474e-b7cd-d34090f80816 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:55:11Z |
| publishDate | 2022 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:e141bc8d-73ea-474e-b7cd-d34090f808162023-08-15T09:26:59ZIn vitro visualization of cell-to-cell interactions between natural killer cells and sensory neuronsBook sectionhttp://purl.org/coar/resource_type/c_1843uuid:e141bc8d-73ea-474e-b7cd-d34090f80816EnglishSymplectic ElementsHumana Press2022Kim, HWDavies, AOh, SBShimasaki, NCell-to-cell interactions between the immune and nervous systems are increasingly recognized for their importance in health and disease. Assessment of cellular neuro-immune interactions can be aided by co-culture of two (or more) cells in an in vitro model system that preserves the morphology of neuronal cells. Here we describe methods to investigate the cytotoxic effector functions of natural killer cells on sensory neurons isolated from syngeneic embryonic and adult mice. We present methods for the morphological analysis of axon fragmentation (pruning) and dynamic cell function via live confocal calcium imaging. These techniques can easily be adapted to study interactions between other combinations of immune cell subsets and neuronal populations. |
| spellingShingle | Kim, HW Davies, A Oh, SB In vitro visualization of cell-to-cell interactions between natural killer cells and sensory neurons |
| title | In vitro visualization of cell-to-cell interactions between natural killer cells and sensory neurons |
| title_full | In vitro visualization of cell-to-cell interactions between natural killer cells and sensory neurons |
| title_fullStr | In vitro visualization of cell-to-cell interactions between natural killer cells and sensory neurons |
| title_full_unstemmed | In vitro visualization of cell-to-cell interactions between natural killer cells and sensory neurons |
| title_short | In vitro visualization of cell-to-cell interactions between natural killer cells and sensory neurons |
| title_sort | in vitro visualization of cell to cell interactions between natural killer cells and sensory neurons |
| work_keys_str_mv | AT kimhw invitrovisualizationofcelltocellinteractionsbetweennaturalkillercellsandsensoryneurons AT daviesa invitrovisualizationofcelltocellinteractionsbetweennaturalkillercellsandsensoryneurons AT ohsb invitrovisualizationofcelltocellinteractionsbetweennaturalkillercellsandsensoryneurons |