Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents.
AIM: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO). METHODS: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl(2)MDP-lip-treated group, n = 10)...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1797099772543238144 |
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| author | Lois, N Dawson, R Townend, J McKinnon, A Smith, G van't Hof, R Van Rooijen, N Forrester, J |
| author_facet | Lois, N Dawson, R Townend, J McKinnon, A Smith, G van't Hof, R Van Rooijen, N Forrester, J |
| author_sort | Lois, N |
| collection | OXFORD |
| description | AIM: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO). METHODS: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl(2)MDP-lip-treated group, n = 10) or phosphate-buffered saline (PBS) (control group, n = 8) 1 day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. The Fisher exact test and randomisation test were used to assess statistically differences between groups. RESULTS: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl(2)MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring ring formation showed no statistically significance between groups (p = 0.27, p = 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the centre of the posterior capsule was found in the Cl(2)MDP-lip-treated group (p = 0.009). CONCLUSION: Depletion of macrophages was accompanied by a reduction in LEC in the centre of the posterior capsule in rodents. |
| first_indexed | 2024-03-07T05:28:21Z |
| format | Journal article |
| id | oxford-uuid:e155c0bf-cbfb-43de-a0a3-9431feaacb01 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:28:21Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:e155c0bf-cbfb-43de-a0a3-9431feaacb012022-03-27T09:53:45ZEffect of short-term macrophage depletion in the development of posterior capsule opacification in rodents.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e155c0bf-cbfb-43de-a0a3-9431feaacb01EnglishSymplectic Elements at Oxford2008Lois, NDawson, RTownend, JMcKinnon, ASmith, Gvan't Hof, RVan Rooijen, NForrester, J AIM: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO). METHODS: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl(2)MDP-lip-treated group, n = 10) or phosphate-buffered saline (PBS) (control group, n = 8) 1 day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. The Fisher exact test and randomisation test were used to assess statistically differences between groups. RESULTS: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl(2)MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring ring formation showed no statistically significance between groups (p = 0.27, p = 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the centre of the posterior capsule was found in the Cl(2)MDP-lip-treated group (p = 0.009). CONCLUSION: Depletion of macrophages was accompanied by a reduction in LEC in the centre of the posterior capsule in rodents. |
| spellingShingle | Lois, N Dawson, R Townend, J McKinnon, A Smith, G van't Hof, R Van Rooijen, N Forrester, J Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. |
| title | Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. |
| title_full | Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. |
| title_fullStr | Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. |
| title_full_unstemmed | Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. |
| title_short | Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. |
| title_sort | effect of short term macrophage depletion in the development of posterior capsule opacification in rodents |
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