High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue.
We have analyzed the V-gene usage in gamma delta T cells of the human gut and joint by using a new mAb (B18) specific for V gamma 8 of human TCR-gamma delta+ T cells. The B18+ population constituted a minor subset of the gamma delta T cells in peripheral blood (PB) of healthy persons (6 +/- 5%) and...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Journal article |
Language: | English |
Published: |
1994
|
_version_ | 1797099777360396288 |
---|---|
author | Söderström, K Bucht, A Halapi, E Lundqvist, C Grönberg, A Nilsson, E Orsini, D van de Wal, Y Koning, F Hammarström, M |
author_facet | Söderström, K Bucht, A Halapi, E Lundqvist, C Grönberg, A Nilsson, E Orsini, D van de Wal, Y Koning, F Hammarström, M |
author_sort | Söderström, K |
collection | OXFORD |
description | We have analyzed the V-gene usage in gamma delta T cells of the human gut and joint by using a new mAb (B18) specific for V gamma 8 of human TCR-gamma delta+ T cells. The B18+ population constituted a minor subset of the gamma delta T cells in peripheral blood (PB) of healthy persons (6 +/- 5%) and only 1 of 35 gamma delta T cell clones analyzed was positive. In contrast, the B18+ subset was a dominant gamma delta T cell population among intraepithelial lymphocytes (IEL) derived from the human intestine (74 +/- 29, p < 0.002), and two of three IEL clones from patients with coeliac disease were B18+. Interestingly, a higher proportion of B18+ gamma delta T cells was found in the synovial fluid of patients with rheumatoid arthritis (RA) (21 +/- 18%, 0.02 < p < 0.05) compared with normal PB. Furthermore, the B18+ subset was more frequent among IL-2-expanded gamma delta T cells (42 +/- 20%) derived from synovial tissue than among IL-2-expanded cells derived from synovial fluid (p < 0.002) and PB from RA patients (p < 0.02) as well as normal PB (p < 0.002). The V-gene usage of 13 gamma delta T cell clones from the synovial fluid of arthritic patients was analyzed. All B18+ clones (n = 7) expressed mRNA for V gamma 8 together with mRNA for V delta 1 (n = 5) or mRNA for V delta 3 (n = 2). None of the B18- clones expressed V gamma 8 (n = 6). We conclude that the gamma delta T cell that expresses V gamma 8, together with mainly V delta 1, is a major gamma delta T cell subset among the IEL of the gut and a highly frequent subset in the synovial tissue of patients with RA. This subset may correspond to the mouse V gamma 7+ IEL, which has a high degree of amino acid sequence homology with the human V gamma 8 protein. |
first_indexed | 2024-03-07T05:28:26Z |
format | Journal article |
id | oxford-uuid:e15c36b1-4502-4330-b31e-24829dd187f9 |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-07T05:28:26Z |
publishDate | 1994 |
record_format | dspace |
spelling | oxford-uuid:e15c36b1-4502-4330-b31e-24829dd187f92022-03-27T09:53:58ZHigh expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e15c36b1-4502-4330-b31e-24829dd187f9EnglishSymplectic Elements at Oxford1994Söderström, KBucht, AHalapi, ELundqvist, CGrönberg, ANilsson, EOrsini, Dvan de Wal, YKoning, FHammarström, MWe have analyzed the V-gene usage in gamma delta T cells of the human gut and joint by using a new mAb (B18) specific for V gamma 8 of human TCR-gamma delta+ T cells. The B18+ population constituted a minor subset of the gamma delta T cells in peripheral blood (PB) of healthy persons (6 +/- 5%) and only 1 of 35 gamma delta T cell clones analyzed was positive. In contrast, the B18+ subset was a dominant gamma delta T cell population among intraepithelial lymphocytes (IEL) derived from the human intestine (74 +/- 29, p < 0.002), and two of three IEL clones from patients with coeliac disease were B18+. Interestingly, a higher proportion of B18+ gamma delta T cells was found in the synovial fluid of patients with rheumatoid arthritis (RA) (21 +/- 18%, 0.02 < p < 0.05) compared with normal PB. Furthermore, the B18+ subset was more frequent among IL-2-expanded gamma delta T cells (42 +/- 20%) derived from synovial tissue than among IL-2-expanded cells derived from synovial fluid (p < 0.002) and PB from RA patients (p < 0.02) as well as normal PB (p < 0.002). The V-gene usage of 13 gamma delta T cell clones from the synovial fluid of arthritic patients was analyzed. All B18+ clones (n = 7) expressed mRNA for V gamma 8 together with mRNA for V delta 1 (n = 5) or mRNA for V delta 3 (n = 2). None of the B18- clones expressed V gamma 8 (n = 6). We conclude that the gamma delta T cell that expresses V gamma 8, together with mainly V delta 1, is a major gamma delta T cell subset among the IEL of the gut and a highly frequent subset in the synovial tissue of patients with RA. This subset may correspond to the mouse V gamma 7+ IEL, which has a high degree of amino acid sequence homology with the human V gamma 8 protein. |
spellingShingle | Söderström, K Bucht, A Halapi, E Lundqvist, C Grönberg, A Nilsson, E Orsini, D van de Wal, Y Koning, F Hammarström, M High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue. |
title | High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue. |
title_full | High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue. |
title_fullStr | High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue. |
title_full_unstemmed | High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue. |
title_short | High expression of V gamma 8 is a shared feature of human gamma delta T cells in the epithelium of the gut and in the inflamed synovial tissue. |
title_sort | high expression of v gamma 8 is a shared feature of human gamma delta t cells in the epithelium of the gut and in the inflamed synovial tissue |
work_keys_str_mv | AT soderstromk highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT buchta highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT halapie highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT lundqvistc highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT gronberga highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT nilssone highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT orsinid highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT vandewaly highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT koningf highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue AT hammarstromm highexpressionofvgamma8isasharedfeatureofhumangammadeltatcellsintheepitheliumofthegutandintheinflamedsynovialtissue |