Replicating landmine blast loading in cellular in vitro models
Trauma arising from landmines and improvised explosive devices promotes heterotopic ossification, the formation of extra-skeletal bone in non-osseous tissue. To date, experimental platforms that can replicate the loading parameter space relevant to improvised explosive device and landmine blast wave...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
IOP Publishing
2020
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| _version_ | 1797109315743514624 |
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| author | Sory, DR Amin, HD Chapman, DJ Proud, WG Rankin, SM |
| author_facet | Sory, DR Amin, HD Chapman, DJ Proud, WG Rankin, SM |
| author_sort | Sory, DR |
| collection | OXFORD |
| description | Trauma arising from landmines and improvised explosive devices promotes heterotopic ossification, the formation of extra-skeletal bone in non-osseous tissue. To date, experimental platforms that can replicate the loading parameter space relevant to improvised explosive device and landmine blast wave exposure have not been available to study the effects of such non-physiological mechanical loading on cells. Here, we present the design and calibration of three distinct in vitro experimental loading platforms that allow us to replicate the spectrum of loading conditions recorded in near-field blast wave exposure. We subjected cells in suspension or in a three-dimensional hydrogel to strain rates up to 6000 s−1 and pressure levels up to 45 MPa. Our results highlight that cellular activation is regulated in a non-linear fashion—not by a single mechanical parameter, it is the combined action of the applied mechanical pressure, rate of loading and loading impulse, along with the extracellular environment used to convey the pressure waves. Finally, our research indicates that PO MSCs are finely tuned to respond to mechanical stimuli that fall within defined ranges of loading. |
| first_indexed | 2024-03-07T07:40:07Z |
| format | Journal article |
| id | oxford-uuid:e20082f6-5618-4fd6-9184-030a75a34c3d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:40:07Z |
| publishDate | 2020 |
| publisher | IOP Publishing |
| record_format | dspace |
| spelling | oxford-uuid:e20082f6-5618-4fd6-9184-030a75a34c3d2023-04-13T11:46:52ZReplicating landmine blast loading in cellular in vitro modelsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e20082f6-5618-4fd6-9184-030a75a34c3dEnglishSymplectic ElementsIOP Publishing2020Sory, DRAmin, HDChapman, DJProud, WGRankin, SMTrauma arising from landmines and improvised explosive devices promotes heterotopic ossification, the formation of extra-skeletal bone in non-osseous tissue. To date, experimental platforms that can replicate the loading parameter space relevant to improvised explosive device and landmine blast wave exposure have not been available to study the effects of such non-physiological mechanical loading on cells. Here, we present the design and calibration of three distinct in vitro experimental loading platforms that allow us to replicate the spectrum of loading conditions recorded in near-field blast wave exposure. We subjected cells in suspension or in a three-dimensional hydrogel to strain rates up to 6000 s−1 and pressure levels up to 45 MPa. Our results highlight that cellular activation is regulated in a non-linear fashion—not by a single mechanical parameter, it is the combined action of the applied mechanical pressure, rate of loading and loading impulse, along with the extracellular environment used to convey the pressure waves. Finally, our research indicates that PO MSCs are finely tuned to respond to mechanical stimuli that fall within defined ranges of loading. |
| spellingShingle | Sory, DR Amin, HD Chapman, DJ Proud, WG Rankin, SM Replicating landmine blast loading in cellular in vitro models |
| title | Replicating landmine blast loading in cellular in vitro models |
| title_full | Replicating landmine blast loading in cellular in vitro models |
| title_fullStr | Replicating landmine blast loading in cellular in vitro models |
| title_full_unstemmed | Replicating landmine blast loading in cellular in vitro models |
| title_short | Replicating landmine blast loading in cellular in vitro models |
| title_sort | replicating landmine blast loading in cellular in vitro models |
| work_keys_str_mv | AT sorydr replicatinglandmineblastloadingincellularinvitromodels AT aminhd replicatinglandmineblastloadingincellularinvitromodels AT chapmandj replicatinglandmineblastloadingincellularinvitromodels AT proudwg replicatinglandmineblastloadingincellularinvitromodels AT rankinsm replicatinglandmineblastloadingincellularinvitromodels |