Protein sequences bound to mineral surfaces persist into deep time.
Proteins persist longer in the fossil record than DNA, but the longevity, survival mechanisms and substrates remain contested. Here, we demonstrate the role of mineral binding in preserving the protein sequence in ostrich (Struthionidae) eggshell, including from the palaeontological sites of Laetoli...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
eLife
2016
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| _version_ | 1826301428300251136 |
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| author | Demarchi, B Hall, S Roncal-Herrero, T Freeman, C Woolley, J Crisp, M Wilson, J Fotakis, A Fischer, R Kessler, B Rakownikow Jersie-Christensen, R Olsen, J Haile, J Thomas, J Marean, C Parkington, J Presslee, S Lee-Thorp, J Ditchfield, P Hamilton, J Ward, M Wang, C Shaw, M Harrison, T Domínguez-Rodrigo, M MacPhee, R Kwekason, A Ecker, M Kolska Horwitz, L Chazan, M Kröger, R Thomas-Oates, J Harding, J Cappellini, E Penkman, K Collins, M |
| author_facet | Demarchi, B Hall, S Roncal-Herrero, T Freeman, C Woolley, J Crisp, M Wilson, J Fotakis, A Fischer, R Kessler, B Rakownikow Jersie-Christensen, R Olsen, J Haile, J Thomas, J Marean, C Parkington, J Presslee, S Lee-Thorp, J Ditchfield, P Hamilton, J Ward, M Wang, C Shaw, M Harrison, T Domínguez-Rodrigo, M MacPhee, R Kwekason, A Ecker, M Kolska Horwitz, L Chazan, M Kröger, R Thomas-Oates, J Harding, J Cappellini, E Penkman, K Collins, M |
| author_sort | Demarchi, B |
| collection | OXFORD |
| description | Proteins persist longer in the fossil record than DNA, but the longevity, survival mechanisms and substrates remain contested. Here, we demonstrate the role of mineral binding in preserving the protein sequence in ostrich (Struthionidae) eggshell, including from the palaeontological sites of Laetoli (3.8 Ma) and Olduvai Gorge (1.3 Ma) in Tanzania. By tracking protein diagenesis back in time we find consistent patterns of preservation, demonstrating authenticity of the surviving sequences. Molecular dynamics simulations of struthiocalcin-1 and -2, the dominant proteins within the eggshell, reveal that distinct domains bind to the mineral surface. It is the domain with the strongest calculated binding energy to the calcite surface that is selectively preserved. Thermal age calculations demonstrate that the Laetoli and Olduvai peptides are 50 times older than any previously authenticated sequence (equivalent to ~16 Ma at a constant10˚C). |
| first_indexed | 2024-03-07T05:32:17Z |
| format | Journal article |
| id | oxford-uuid:e2ae54c4-3050-404a-9477-1e9ede7b4abb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:32:17Z |
| publishDate | 2016 |
| publisher | eLife |
| record_format | dspace |
| spelling | oxford-uuid:e2ae54c4-3050-404a-9477-1e9ede7b4abb2022-03-27T10:03:19ZProtein sequences bound to mineral surfaces persist into deep time.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e2ae54c4-3050-404a-9477-1e9ede7b4abbEnglishSymplectic Elements at OxfordeLife2016Demarchi, BHall, SRoncal-Herrero, TFreeman, CWoolley, JCrisp, MWilson, JFotakis, AFischer, RKessler, BRakownikow Jersie-Christensen, ROlsen, JHaile, JThomas, JMarean, CParkington, JPresslee, SLee-Thorp, JDitchfield, PHamilton, JWard, MWang, CShaw, MHarrison, TDomínguez-Rodrigo, MMacPhee, RKwekason, AEcker, MKolska Horwitz, LChazan, MKröger, RThomas-Oates, JHarding, JCappellini, EPenkman, KCollins, MProteins persist longer in the fossil record than DNA, but the longevity, survival mechanisms and substrates remain contested. Here, we demonstrate the role of mineral binding in preserving the protein sequence in ostrich (Struthionidae) eggshell, including from the palaeontological sites of Laetoli (3.8 Ma) and Olduvai Gorge (1.3 Ma) in Tanzania. By tracking protein diagenesis back in time we find consistent patterns of preservation, demonstrating authenticity of the surviving sequences. Molecular dynamics simulations of struthiocalcin-1 and -2, the dominant proteins within the eggshell, reveal that distinct domains bind to the mineral surface. It is the domain with the strongest calculated binding energy to the calcite surface that is selectively preserved. Thermal age calculations demonstrate that the Laetoli and Olduvai peptides are 50 times older than any previously authenticated sequence (equivalent to ~16 Ma at a constant10˚C). |
| spellingShingle | Demarchi, B Hall, S Roncal-Herrero, T Freeman, C Woolley, J Crisp, M Wilson, J Fotakis, A Fischer, R Kessler, B Rakownikow Jersie-Christensen, R Olsen, J Haile, J Thomas, J Marean, C Parkington, J Presslee, S Lee-Thorp, J Ditchfield, P Hamilton, J Ward, M Wang, C Shaw, M Harrison, T Domínguez-Rodrigo, M MacPhee, R Kwekason, A Ecker, M Kolska Horwitz, L Chazan, M Kröger, R Thomas-Oates, J Harding, J Cappellini, E Penkman, K Collins, M Protein sequences bound to mineral surfaces persist into deep time. |
| title | Protein sequences bound to mineral surfaces persist into deep time. |
| title_full | Protein sequences bound to mineral surfaces persist into deep time. |
| title_fullStr | Protein sequences bound to mineral surfaces persist into deep time. |
| title_full_unstemmed | Protein sequences bound to mineral surfaces persist into deep time. |
| title_short | Protein sequences bound to mineral surfaces persist into deep time. |
| title_sort | protein sequences bound to mineral surfaces persist into deep time |
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