Proteomic analysis of microtubule-associated proteins during macrophage activation.
Classical activation of macrophages induces a wide range of signaling and vesicle trafficking events to produce a more aggressive cellular phenotype. The microtubule (MT) cytoskeleton is crucial for the regulation of immune responses. In the current study, we used a large scale proteomics approach t...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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2009
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| _version_ | 1826301428884307968 |
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| author | Patel, P Fisher, K Yang, E Deane, C Harrison, R |
| author_facet | Patel, P Fisher, K Yang, E Deane, C Harrison, R |
| author_sort | Patel, P |
| collection | OXFORD |
| description | Classical activation of macrophages induces a wide range of signaling and vesicle trafficking events to produce a more aggressive cellular phenotype. The microtubule (MT) cytoskeleton is crucial for the regulation of immune responses. In the current study, we used a large scale proteomics approach to analyze the change in protein composition of the MT-associated protein (MAP) network by macrophage stimulation with the inflammatory cytokine interferon-gamma and the endotoxin lipopolysaccharide. Overall the analysis identified 409 proteins that bound directly or indirectly to MTs. Of these, 52 were up-regulated 2-fold or greater and 42 were down-regulated 2-fold or greater after interferon-gamma/lipopolysaccharide stimulation. Bioinformatics analysis based on publicly available binary protein interaction data produced a putative interaction network of MAPs in activated macrophages. We confirmed the up-regulation of several MAPs by immunoblotting and immunofluorescence analysis. More detailed analysis of one up-regulated protein revealed a role for HSP90beta in stabilization of the MT cytoskeleton during macrophage activation. |
| first_indexed | 2024-03-07T05:32:18Z |
| format | Journal article |
| id | oxford-uuid:e2af0ba6-e0bb-4d0b-8ba6-33b4ca8b73e9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:32:18Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:e2af0ba6-e0bb-4d0b-8ba6-33b4ca8b73e92022-03-27T10:03:11ZProteomic analysis of microtubule-associated proteins during macrophage activation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e2af0ba6-e0bb-4d0b-8ba6-33b4ca8b73e9EnglishSymplectic Elements at Oxford2009Patel, PFisher, KYang, EDeane, CHarrison, RClassical activation of macrophages induces a wide range of signaling and vesicle trafficking events to produce a more aggressive cellular phenotype. The microtubule (MT) cytoskeleton is crucial for the regulation of immune responses. In the current study, we used a large scale proteomics approach to analyze the change in protein composition of the MT-associated protein (MAP) network by macrophage stimulation with the inflammatory cytokine interferon-gamma and the endotoxin lipopolysaccharide. Overall the analysis identified 409 proteins that bound directly or indirectly to MTs. Of these, 52 were up-regulated 2-fold or greater and 42 were down-regulated 2-fold or greater after interferon-gamma/lipopolysaccharide stimulation. Bioinformatics analysis based on publicly available binary protein interaction data produced a putative interaction network of MAPs in activated macrophages. We confirmed the up-regulation of several MAPs by immunoblotting and immunofluorescence analysis. More detailed analysis of one up-regulated protein revealed a role for HSP90beta in stabilization of the MT cytoskeleton during macrophage activation. |
| spellingShingle | Patel, P Fisher, K Yang, E Deane, C Harrison, R Proteomic analysis of microtubule-associated proteins during macrophage activation. |
| title | Proteomic analysis of microtubule-associated proteins during macrophage activation. |
| title_full | Proteomic analysis of microtubule-associated proteins during macrophage activation. |
| title_fullStr | Proteomic analysis of microtubule-associated proteins during macrophage activation. |
| title_full_unstemmed | Proteomic analysis of microtubule-associated proteins during macrophage activation. |
| title_short | Proteomic analysis of microtubule-associated proteins during macrophage activation. |
| title_sort | proteomic analysis of microtubule associated proteins during macrophage activation |
| work_keys_str_mv | AT patelp proteomicanalysisofmicrotubuleassociatedproteinsduringmacrophageactivation AT fisherk proteomicanalysisofmicrotubuleassociatedproteinsduringmacrophageactivation AT yange proteomicanalysisofmicrotubuleassociatedproteinsduringmacrophageactivation AT deanec proteomicanalysisofmicrotubuleassociatedproteinsduringmacrophageactivation AT harrisonr proteomicanalysisofmicrotubuleassociatedproteinsduringmacrophageactivation |