CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflamm...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826301448436056064 |
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| author | Hyde, S Pringle, I Abdullah, S Lawton, A Davies, L Varathalingam, A Nunez-Alonso, G Green, A Bazzani, R Sumner-Jones, S Chan, M Li, H Yew, N Cheng, S Boyd, A Davies, J Griesenbach, U Porteous, D Sheppard, D Munkonge, F Alton, E Gill, D |
| author_facet | Hyde, S Pringle, I Abdullah, S Lawton, A Davies, L Varathalingam, A Nunez-Alonso, G Green, A Bazzani, R Sumner-Jones, S Chan, M Li, H Yew, N Cheng, S Boyd, A Davies, J Griesenbach, U Porteous, D Sheppard, D Munkonge, F Alton, E Gill, D |
| author_sort | Hyde, S |
| collection | OXFORD |
| description | Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation. |
| first_indexed | 2024-03-07T05:32:34Z |
| format | Journal article |
| id | oxford-uuid:e2c6fa2d-6ce3-4bd4-a1a2-177fd0f70cbf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T05:32:34Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:e2c6fa2d-6ce3-4bd4-a1a2-177fd0f70cbf2022-03-27T10:04:02ZCpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:e2c6fa2d-6ce3-4bd4-a1a2-177fd0f70cbfEnglishSymplectic Elements at Oxford2008Hyde, SPringle, IAbdullah, SLawton, ADavies, LVarathalingam, ANunez-Alonso, GGreen, ABazzani, RSumner-Jones, SChan, MLi, HYew, NCheng, SBoyd, ADavies, JGriesenbach, UPorteous, DSheppard, DMunkonge, FAlton, EGill, DPulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation. |
| spellingShingle | Hyde, S Pringle, I Abdullah, S Lawton, A Davies, L Varathalingam, A Nunez-Alonso, G Green, A Bazzani, R Sumner-Jones, S Chan, M Li, H Yew, N Cheng, S Boyd, A Davies, J Griesenbach, U Porteous, D Sheppard, D Munkonge, F Alton, E Gill, D CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_full | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_fullStr | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_full_unstemmed | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_short | CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. |
| title_sort | cpg free plasmids confer reduced inflammation and sustained pulmonary gene expression |
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